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Literature summary for 4.8.1.3 extracted from
- Indoleacetaldoxime hydro-lyase. II. Purification and properties (1968), Arch. Biochem. Biophys., 125, 873-883.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | activates | Fusarium fujikuroi |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Dimercaptopropanol | - |
Fusarium fujikuroi | |
| 2-mercaptoethanol | - |
Fusarium fujikuroi | |
| 8-hydroxyquinoline | inhibition is partly reversed by ferric citrate, ascorbic acid and dehydroascorbic acid | Fusarium fujikuroi | |
| Ag+ | 1 mM, complete inactivation | Fusarium fujikuroi | |
| Al3+ | 1 mM, complete inactivation | Fusarium fujikuroi | |
| Benzaldoxime | - |
Fusarium fujikuroi | |
| Cu2+ | 1 mM, complete inactivation | Fusarium fujikuroi | |
| Cys | - |
Fusarium fujikuroi | |
| Diethyl dithiocarbamate | - |
Fusarium fujikuroi | |
| Hg2+ | 1 mM, complete inactivation | Fusarium fujikuroi | |
| KCN | activates at 0.001-0.1 mM, inhibits at 1-10 mM | Fusarium fujikuroi | |
| Mandelaldoxime | - |
Fusarium fujikuroi | |
| Mercaptoacetic acid | weak | Fusarium fujikuroi | |
| Mo5+ | 1 mM, complete inactivation | Fusarium fujikuroi | |
| NaBH4 | inhibition is partly reversed by pyridoxal-5'-phosphate or dehydroascorbic acid | Fusarium fujikuroi | |
| NEM | - |
Fusarium fujikuroi | |
| p-hydroxymercuribenzoate | - |
Fusarium fujikuroi | |
| phenylacetaldoxime | competitive | Fusarium fujikuroi | |
| Phenylpropionaldoxime | - |
Fusarium fujikuroi | |
| Zn2+ | 1 mM, complete inactivation | Fusarium fujikuroi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
3-indoleacetaldoxime | - |
Fusarium fujikuroi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | Ferric citrate promotes activity | Fusarium fujikuroi | |
| KCN | activates at 0.001-0.1 mM, inhibits at 1-10 mM | Fusarium fujikuroi | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium fujikuroi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fusarium fujikuroi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Fusarium fujikuroi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-Indoleacetaldoxime | - |
Fusarium fujikuroi | 3-Indoleacetonitrile | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Fusarium fujikuroi |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | pH 6.0: about 60% of maximal activity, pH 9.0: about 35% of maximal activity | Fusarium fujikuroi |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | maximally stable | Fusarium fujikuroi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | required | Fusarium fujikuroi | |
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