Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | the enzyme activity is enhanced 1.8fold by 1 mM | Rhodococcus sp. N-771 | |
cysteamine | the enzyme activity is enhanced 2.3fold by 1 mM | Rhodococcus sp. N-771 | |
duroquinone | the enzyme activity is increased 6.7fold in the absence of Na2S | Rhodococcus sp. N-771 | |
FAD | the enzyme activity is enhanced 3fold by 1 mM | Rhodococcus sp. N-771 | |
FMN | the enzyme activity is enhanced 7.2fold by 1 mM | Rhodococcus sp. N-771 | |
L-cysteine | the enzyme activity is enhanced 2.4fold by 1 mM | Rhodococcus sp. N-771 | |
additional information | the activity is not increased by the additions of NaHSO3, Na2SO4, NaHSO4or Na2S2O7. The following coenzymes do not affect the reaction: pantothenate, phosphoenolpyruvate, NaF, pyridoxal, dehydroascorbate, glutathione, glutathione disulfide, NAD(P)(H), D-glucose 6-phosphate, glutathione disulfide, dehydroascorbic acid, CoA-SH, AXP, GXP, and IXP | Rhodococcus sp. N-771 | |
Na2S | the enzyme activity is enhanced 4.5fold by 1 mM | Rhodococcus sp. N-771 | |
Na2S2O4 | the enzyme activity is enhanced 2.3fold by 1 mM | Rhodococcus sp. N-771 | |
Na2S2O5 | the enzyme activity is enhanced 13.8fold by 1 mM | Rhodococcus sp. N-771 | |
Na2SO3 | the enzyme activity is enhanced 5.4fold by 1 mM | Rhodococcus sp. N-771 | |
riboflavin | the enzyme activity is enhanced 3.4fold by 1 mM | Rhodococcus sp. N-771 | |
thioglycerol | the enzyme activity is enhanced 3.5fold by 1 mM | Rhodococcus sp. N-771 | |
vitamin K3 | the enzyme activity is increased 23fold in the presence of Na2S | Rhodococcus sp. N-771 |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Star (DE3) cells | Rhodococcus sp. N-771 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(E)-benzaldoxime | - |
Rhodococcus sp. N-771 | |
2-phenylenediamine | slight inhibition in the absence of Na2S | Rhodococcus sp. N-771 | |
acetophenone | - |
Rhodococcus sp. N-771 | |
As3+ | - |
Rhodococcus sp. N-771 | |
Cd2+ | - |
Rhodococcus sp. N-771 | |
Cu+ | - |
Rhodococcus sp. N-771 | |
Cu2+ | - |
Rhodococcus sp. N-771 | |
D-cycloserine | 35% inhibition at 0.1 mM in the absence of Na2S | Rhodococcus sp. N-771 | |
digallol | slight inhibition in the absence of Na2S | Rhodococcus sp. N-771 | |
EDTA | 45% inhibition at 0.1 mM in the absence of Na2S | Rhodococcus sp. N-771 | |
guaiacol | slight inhibition in the absence of Na2S | Rhodococcus sp. N-771 | |
Hg2+ | - |
Rhodococcus sp. N-771 | |
additional information | not inhibited by hydrazine | Rhodococcus sp. N-771 | |
phenylacetone | - |
Rhodococcus sp. N-771 | |
phenylhydrazine | - |
Rhodococcus sp. N-771 | |
Tiron | 55% inhibition at 0.1 mM in the absence of Na2S | Rhodococcus sp. N-771 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the enzyme activity is increased 14fold by 1 mM Co2+ | Rhodococcus sp. N-771 | |
D-cycloserine | the enzyme activity is increased 1.6fold at 0.1 mM D-cycloserine in the presence of Na2S | Rhodococcus sp. N-771 | |
EDTA | the enzyme activity is increased 2.1fold at 0.1 mM EDTA in the presence of Na2S | Rhodococcus sp. N-771 | |
Fe2+ | the enzyme activity is increased 23.5fold by 1 mM Fe2+ | Rhodococcus sp. N-771 | |
Fe3+ | the enzyme activity is increased 24.3fold by 1 mM Fe3+ | Rhodococcus sp. N-771 | |
additional information | the following metal ions do not inhibit the enzyme activity under any reaction conditions: Li+, B4+, Na+, Mg2+, K+, Ca2+, Mn2+, Ti2+, Cr3+, Rb+, Mo6+, Ag+, Cs+, Ba2+ and Pb2+ | Rhodococcus sp. N-771 | |
Sn2+ | the enzyme activity is increased 3.6fold by 1 mM Sn2+ | Rhodococcus sp. N-771 | |
Tiron | the enzyme activity is increased 7.2fold at 0.1 mM Tiron in the presence of Na2S | Rhodococcus sp. N-771 | |
Zn2+ | the enzyme activity is increased 2.5fold by 1 mM Zn2+ | Rhodococcus sp. N-771 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Rhodococcus sp. N-771 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an aliphatic aldoxime | Rhodococcus sp. N-771 | the enzyme preferentially acts on aliphatic-type substrates | an aliphatic nitrile + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. N-771 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE column chromatography, butyl-Toyopearl column chromatography and Superdex 200 gel filtration | Rhodococcus sp. N-771 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.92 | - |
after 7.1fold purification, at pH 8.0 and 30°C | Rhodococcus sp. N-771 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-4-chlorobenzaldoxime | 0.331% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | ? | - |
? | |
(E)-4-tolualdoxime | 0.488% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | ? | - |
? | |
(E)-benzaldoxime | 1.92% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | ? | - |
? | |
(E)-furfurylaldoxime | 0.504% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | ? | - |
? | |
(E)-pyridine-3-aldoxime | 0.535% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | ? | - |
? | |
(E)-thiophene-2-acetaldoxime | 26.3% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | ? | - |
? | |
(E)-thiophene-2-carboxaldoxime | 3.37% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | ? | - |
? | |
(E/Z)-2-phenylpropionaldoxime | 49.8% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | (E/Z)-2-phenylpropiononitrile + H2O | - |
? | |
(E/Z)-4-phenylbutyraldoxime | 29.1% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | phenylbutyronitrile + H2O | - |
? | |
(E/Z)-cinnamaldehyde oxime | 4.28% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | ? | - |
? | |
(E/Z)-cyclohexanecarboxaldehyde oxime | 157% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | ? | - |
? | |
(E/Z)-indoleacetaldoxime | 41.7% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | indoleacetonitrile + H2O | - |
? | |
(E/Z)-isobutyraldoxime | 89.6% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | ? | - |
? | |
(E/Z)-isocapronaldoxime | 48.7% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | isocapronitrile + H2O | - |
? | |
(E/Z)-isovaleraldoxime | 120% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | isovaleronitrile + H2O | - |
? | |
(E/Z)-mandelaldoxime | 11.7% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | mandeloacetonitrile + H2O | - |
? | |
(E/Z)-n-butyraldoxime | 100% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | n-butyronitrile + H2O | - |
? | |
(E/Z)-n-capronaldoxime | 85.5% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | n-caprononitrile + H2O | - |
? | |
(E/Z)-n-valeraldoxime | 68.3% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | n-valeronitrile + H2O | - |
? | |
(E/Z)-propionaldoxime | 87.7% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | propionitrile + H2O | - |
? | |
(Z)-3-phenylpropionaldoxime | 69.6% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | 3-phenylpropiononitrile | - |
? | |
(Z)-4-chlorophenylacetaldoxime | 3.39% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | 4-chlorophenylacetonitrile + H2O | - |
? | |
(Z)-naphthoacetaldoxime | 2.71% activity in the presence of Na2S compared to (Z)-phenylacetaldehyde oxime | Rhodococcus sp. N-771 | naphthoacetonitrile + H2O | - |
? | |
(Z)-phenylacetaldehyde oxime | 100% activity in the presence of Na2S | Rhodococcus sp. N-771 | phenylacetonitrile + H2O | - |
? | |
an aliphatic aldoxime | the enzyme preferentially acts on aliphatic-type substrates | Rhodococcus sp. N-771 | an aliphatic nitrile + H2O | - |
? | |
additional information | the following compounds are inert as substrates: ((E/Z))-diphenylacetaldoxime, ((E/Z))-4-hydroxyphenylacetaldoxime, (E)-1-naphthoaldoxime, (E)-anisaldoxime, (E)-quinolin(E)-2-carboxaldehyde oxime, (E)-terephthalaldehyde oxime, (E)-isophthalaldehyde oxime, (E)-pyrazinecarboxaldoxime, (E)-indol(E)-3-carboxaldehyde oxime, (Z)-crotonaldoxime, ((E/Z))-methacrylaldoxime, ((E/Z))-O-benzylphenylacetaldehyde oxime, (E)-phenylacetaldehyde oxime hydrazone, ((E/Z))-O-acetyl-phenylacetaldehyde oxime, ((E/Z))-phenylacetone oxime and ((E/Z))-acetophenone oxime | Rhodococcus sp. N-771 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 39868, calculated from amino acid sequence | Rhodococcus sp. N-771 |
Synonyms | Comment | Organism |
---|---|---|
OxdRE | - |
Rhodococcus sp. N-771 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Rhodococcus sp. N-771 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rhodococcus sp. N-771 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme b | - |
Rhodococcus sp. N-771 |