Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 2.13fold | Rhodococcus globerulus | |
cysteamine | activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 3.93fold | Rhodococcus globerulus | |
duroquinone | activates | Rhodococcus globerulus | |
FAD | 1 mM, 1.2fold activation in presence of Na2S, 4.5fold in absence of Na2S | Rhodococcus globerulus | |
FMN | 1 mM, 1.7fold activation in presence of Na2S, 2.4fold in absence of Na2S | Rhodococcus globerulus | |
L-cysteine | activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 2.39fold | Rhodococcus globerulus | |
additional information | not activated by NaHSO3, Na2SO4, NaHSO4 or Na2S2O7 | Rhodococcus globerulus | |
Na2S | activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 8.35fold | Rhodococcus globerulus | |
Na2S2O4 | activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 8.13fold | Rhodococcus globerulus | |
Na2S2O5 | activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 5.84fold | Rhodococcus globerulus | |
Na2SO3 | activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 7.51fold | Rhodococcus globerulus | |
reducing reagent | requirement, activates | Rhodococcus globerulus | |
riboflavin | 1 mM, 1.1fold activation in presence of Na2S, 4.2fold in absence of Na2S | Rhodococcus globerulus | |
thioglycerol | activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 4.06fold | Rhodococcus globerulus | |
vitamin K3 | activates | Rhodococcus globerulus |
Application | Comment | Organism |
---|---|---|
synthesis | may be a useful biocatalyst for the production of various nitriles from the corresponding aldoximes | Rhodococcus globerulus |
Cloned (Comment) | Organism |
---|---|
oxd gene, overexpression in Escherichia coli JM109, sequencing, gene cluster organisation | Rhodococcus globerulus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,2,3-benzenetriol | slight inhibition | Rhodococcus globerulus | |
Cd2+ | strong inhibition at 1 mM, activates at 0.1 mM | Rhodococcus globerulus | |
Co2+ | strong inhibition at 1 mM, activates at 0.1 mM | Rhodococcus globerulus | |
Cu+ | strong inhibition at 1 mM, activates at 0.1 mM | Rhodococcus globerulus | |
Cu2+ | strong inhibition at 1 mM, activates at 0.1 mM | Rhodococcus globerulus | |
dimethylphenylenediamine | slight inhibition | Rhodococcus globerulus | |
guaiacol | slight inhibition | Rhodococcus globerulus | |
heavy metal ion | 1 mM | Rhodococcus globerulus | |
miconazole | slight inhibition | Rhodococcus globerulus | |
additional information | not inhibited by hydrazine, KCN, NaN3, and NH2OH | Rhodococcus globerulus | |
nitroblue tetrazolium | slight inhibition | Rhodococcus globerulus | |
p-phenylenediamine | slight inhibition | Rhodococcus globerulus | |
phenazine methosulfate | slight inhibition | Rhodococcus globerulus | |
phenylhydrazine | inhibits dehydration of (Z)-3-phenylpropionaldoxime, but not of (E/Z)-n-valeraldoxime | Rhodococcus globerulus | |
sulfhydryl reagent | 1 mM | Rhodococcus globerulus | |
tetramethylphenylenediamine | slight inhibition | Rhodococcus globerulus | |
Tiron | inhibits dehydration of (Z)-3-phenylpropionaldoxime, but not of (E/Z)-n-valeraldoxime | Rhodococcus globerulus | |
trimethylhydroquinone | slight inhibition | Rhodococcus globerulus | |
Zn2+ | strong inhibition at 1 mM, activates at 0.1 mM | Rhodococcus globerulus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for the activating Na2S is 0.0408 mM | Rhodococcus globerulus | |
1.13 | - |
(E/Z)-n-valeraldoxime | pH 7, 30°C | Rhodococcus globerulus | |
1.13 | - |
(E/Z)-cyclohexanecarboxaldehyde oxime | pH 7, 30°C | Rhodococcus globerulus | |
1.4 | - |
(Z)-phenylacetaldoxime | pH 7, 30°C | Rhodococcus globerulus | |
1.73 | - |
(E/Z)-n-butyraldoxime | pH 7, 30°C | Rhodococcus globerulus | |
2.31 | - |
(Z)-3-phenylpropionaldoxime | pH 7, 30°C | Rhodococcus globerulus | |
2.94 | - |
(E/Z)-n-capronaldoxime | pH 7, 30°C | Rhodococcus globerulus | |
3.23 | - |
(E/Z)-mandelaldoxime | pH 7, 30°C | Rhodococcus globerulus | |
3.91 | - |
(E/Z)-indoleacetaldoxime | pH 7, 30°C | Rhodococcus globerulus | |
3.97 | - |
(E/Z)-isovaleraldoxime | pH 7, 30°C | Rhodococcus globerulus | |
5.13 | - |
(E/Z)-propionaldoxime | pH 7, 30°C | Rhodococcus globerulus | |
5.54 | - |
(E/Z)-isobutyraldoxime | pH 7, 30°C | Rhodococcus globerulus | |
6.76 | - |
(E/Z)-isocapronaldoxime | pH 7, 30°C | Rhodococcus globerulus | |
11.9 | - |
(E/Z)-2-phenylpropionaldoxime | pH 7, 30°C | Rhodococcus globerulus | |
20 | - |
(E)-pyridine-3-aldoxime | pH 7, 30°C | Rhodococcus globerulus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | activates at 0.1 mM, strong inhibition at 1 mM | Rhodococcus globerulus | |
Co2+ | activates at 0.1 mM, strong inhibition at 1 mM | Rhodococcus globerulus | |
Cu+ | activates at 0.1 mM, strong inhibition at 1 mM | Rhodococcus globerulus | |
Cu2+ | activates at 0.1 mM, strong inhibition at 1 mM | Rhodococcus globerulus | |
Fe2+ | heme iron is present in a reduced form, 1 mM, activates | Rhodococcus globerulus | |
Fe3+ | 1 mM, activates | Rhodococcus globerulus | |
Zn2+ | activates at 0.1 mM, strong inhibition at 1 mM | Rhodococcus globerulus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39892 | - |
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence | Rhodococcus globerulus |
42000 | - |
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence | Rhodococcus globerulus |
76200 | - |
wild-type OxdRG, gel filtration | Rhodococcus globerulus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alkylaldoxime | Rhodococcus globerulus | responsible for the metabolism of alkylaldoxime | alkylnitrile + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus globerulus | Q76EV4 | - |
- |
Rhodococcus globerulus A-4 | Q76EV4 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type OxdRG: 104fold, recombinant OxdRG expressed in Escherichia coli JM109: 14.9fold | Rhodococcus globerulus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0879 | - |
pH 7, 30°C, wild-type OxdRG, (Z)-phenylacetaldoxime as substrate, in presence of 1 mM Na2S | Rhodococcus globerulus |
2.18 | - |
pH 7, 30°C, recombinant OxdRG, (Z)-phenylacetaldoxime as substrate, in presence of 1 mM Na2S | Rhodococcus globerulus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-pyridine-3-aldoxime | not Z-form, poor substrate, 0.78% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | pyridine-3-nitrile + H2O | - |
? | |
(E/Z)-2-phenylpropionaldoxime | 5.23% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | 2-phenylpropiononitrile + H2O | - |
? | |
(E/Z)-cyclohexanecarboxaldehyde oxime | best substrate | Rhodococcus globerulus | ? | - |
? | |
(E/Z)-cyclohexanecarboxaldehyde oxime | best substrate | Rhodococcus globerulus A-4 | ? | - |
? | |
(E/Z)-indoleacetaldoxime | 7.29% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | indoleacetonitrile + H2O | - |
? | |
(E/Z)-isobutyraldoxime | 26.5% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | isobutyronitrile + H2O | - |
? | |
(E/Z)-isocapronaldoxime | 88.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | isocaprononitrile + H2O | - |
? | |
(E/Z)-isovaleraldoxime | 58.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | isovaleronitrile + H2O | - |
? | |
(E/Z)-mandelaldoxime | 3.09% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | mandelonitrile + H2O | - |
? | |
(E/Z)-n-butyraldoxime | 48.9% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | n-butyronitrile + H2O | - |
? | |
(E/Z)-n-butyraldoxime | 48.9% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus A-4 | n-butyronitrile + H2O | - |
? | |
(E/Z)-n-capronaldoxime | 64.2% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | n-caprononitrile + H2O | - |
? | |
(E/Z)-n-valeraldoxime | 39.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | n-valeronitrile + H2O | - |
? | |
(E/Z)-n-valeraldoxime | 39.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus A-4 | n-valeronitrile + H2O | - |
? | |
(E/Z)-propionaldoxime | 7.76% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | propiononitrile + H2O | - |
? | |
(Z)-3-phenylpropionaldoxime | 67.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | 3-phenylpropiononitrile + H2O | - |
? | |
(Z)-phenylacetaldoxime | 26.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus | phenylacetonitrile + H2O | - |
? | |
(Z)-phenylacetaldoxime | 26.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime | Rhodococcus globerulus A-4 | phenylacetonitrile + H2O | - |
? | |
aldoxime | wide range of substrates, aliphatic aldoximes are more effective substrates than arylalkyl aldoximes, aromatic aldoximes are also dehydrated | Rhodococcus globerulus | nitrile + H2O | - |
? | |
aldoxime | wide range of substrates, aliphatic aldoximes are more effective substrates than arylalkyl aldoximes, aromatic aldoximes are also dehydrated | Rhodococcus globerulus A-4 | nitrile + H2O | - |
? | |
alkylaldoxime | responsible for the metabolism of alkylaldoxime | Rhodococcus globerulus | alkylnitrile + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence | Rhodococcus globerulus |
Synonyms | Comment | Organism |
---|---|---|
alkylaldoxime dehydratase | - |
Rhodococcus globerulus |
OxdRG | - |
Rhodococcus globerulus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
around, 0.1 M potassium phosphate buffer, pH 8 | Rhodococcus globerulus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, 0.1 M potassium phosphate buffer, pH 8, 35% loss of activity | Rhodococcus globerulus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
0.1 M potassium phosphate buffer | Rhodococcus globerulus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme b | prosthetic group, protoheme IX, heme iron is present in a reduced form, contains 0.37 mol heme per mol of enzyme | Rhodococcus globerulus | |
protoheme IX | prosthetic group, heme b, heme iron is present in a reduced form, contains 0.37 mol heme per mol of enzyme | Rhodococcus globerulus |