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Literature summary for 4.6.1.25 extracted from

  • Saida, F.; Odaert, B.; Uzan, M.; Bontems, F.
    First structural investigation of the restriction ribonuclease RegB NMR spectroscopic conditions, 13C/15N double-isotopic labelling and two-dimensional heteronuclear spectra (2004), Protein Expr. Purif., 34, 158-165 .
    View publication on PubMed
Search for more literature for 4.6.1.25

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Tequatrovirus T4

Protein Variants

Protein Variants Comment Organism
H48A mutant enzyme binds its RNA substrate with the same affinity as the wild type protein. The beta-sheet content of the RegB H48A secondary structure is probably poor but not nil Tequatrovirus T4

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a [pre-mRNA]-containing guanosine-adenosine + H2O Tequatrovirus T4
-
 a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4 P13312
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tequatrovirus T4

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a [pre-mRNA]-containing guanosine-adenosine + H2O
-
 Tequatrovirus T4 a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate
-
 ?
a [pre-mRNA]-containing guanosine-adenosine + H2O the enzyme cleaves with an almost absolute specificity its RNA substrate in the middle of the GGAG tetranucleotide mainly found in the Shine-Dalgarno sequence Tequatrovirus T4 a 5' hydroxy-guanosine-[pre-mRNA fragment] + a [pre-mRNA fragment]-3'-adenosine-3'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
RegB
-
 Tequatrovirus T4

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
32
-
 1.2 mM samples of RegB H48A precipitate at 32.5°C within 18 h. In the presence of sodium citrate molecules no visible precipitation after a 3 days-incubation Tequatrovirus T4