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Literature summary for 4.6.1.24 extracted from

  • Kobayashi, H.; Motoyoshi, N.; Itagaki, T.; Tabata, K.; Suzuki, T.; Inokuchi, N.
    The inhibition of human tumor cell proliferation by RNase Pol, a member of the RNase T1 family, from Pleurotus ostreatus (2013), Biosci. Biotechnol. Biochem., 77, 1486-1491.
    View publication on PubMed
Search for more literature for 4.6.1.24

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)pLysS Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus B1Q4S7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3)pLysS to homogeneity by ammonium sulfate fractionation, gel filtration, cation exchange and anion exchange chromatography, and again gel filtration, heparin affinity chromatography, and dialysis Pleurotus ostreatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2350
-
 purified recombinant enzyme, pH 7.5, 37°C Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information assay substrate is commercial yeast RNA, the enzyme is a guanylic acid-specific RNase Pleurotus ostreatus ?
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 ?

Synonyms

Synonyms Comment Organism
RNase Pol
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 Pleurotus ostreatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Pleurotus ostreatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 purified recombinant enzyme, 5 min, over 80% activity remaining Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Pleurotus ostreatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 11 purified recombinant enzyme, stable Pleurotus ostreatus

General Information

General Information Comment Organism
evolution the enzyme belongs to the RNase T1 family Pleurotus ostreatus
physiological function the enzyme from Pleurotus ostreatus inhibits human tumor cell proliferation, e.g. of neuroblastoma cell lines IMR-32 and SK-N-SH and leukemia cell lines HL-60 and Jurkat. The enzyme causes a sub-G1-cell population formation in HL-60 cells, overview Pleurotus ostreatus