Literature summary for 4.6.1.24 extracted from

Zeller, M.E.; Csanadi, A.; Miczak, A.; Rose, T.; Bizebard, T.; Kaberdin, V.R.
Quaternary structure and biochemical properties of mycobacterial RNase E/G (2007), Biochem. J., 403, 207-215.

Search for more literature for 4.6.1.24

Cloned(Commentary)

Cloned (Comment)	Organism
a 1.8 kb DNA fragment, encoding the C-terminal portion of the MycRne polypeptide, residues 332-953, is cloned into p6HisF-11d for expression in Escherichia coli BL21-CodonPlusDE3-RIL cells	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
71100	-	apparent molecular mass of the monomer, determined by sedimentation equilibrium analysis	Mycobacterium tuberculosis
325000	-	tetramer, determined by gel-filtration	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
usung a HisTrap HP column	Mycobacterium tuberculosis

Storage Stability

Storage Stability	Organism
-80°C, HEPES buffer, pH 7.5, 0.1 mM EDTA, 1 mM DTT, 50 mM NaCl	Mycobacterium tuberculosis

Subunits

Subunits	Comment	Organism
dimer	-	Mycobacterium tuberculosis
tetramer	-	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
MycRne	-	Mycobacterium tuberculosis
RNase E/G	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)