Crystallization (Comment) | Organism |
---|---|
crystal structure of RNase T1-T2 (K41E/Y42F/N43R/Y45W/E46N/W59Y), hanging drop vapor diffusion method, 2.1 A resolution | Aspergillus oryzae |
Protein Variants | Comment | Organism |
---|---|---|
K41E/Y42F/N43R/Y45W/E46N/W59Y | RNase T1-R2 contains the additional mutation W59Y compared to RNase T1-RV variant. RNase T1-RV is a variant where the specificity is changed from guanine to purine, accompanied with a reduced activity. The additional mutation W59Y increases the activity in comparison to variant RV to 425% | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyne K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | P00651 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenylyl-3',5'-cytidine + H2O | kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyme K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) | Aspergillus oryzae | adenosine 3'-phosphate + cytidine | - |
? | |
guanylyl-3',5'-cytidine + H2O | kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyme K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) | Aspergillus oryzae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase T1-R2 | mutant RNase T1 K41E/Y42F/N43R/Y45W/E46N/W59Y | Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyne K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) | Aspergillus oryzae |