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Literature summary for 4.6.1.24 extracted from

  • Fields, R.; Dixon, H.B.F.; Law, G.R.; Yui, C.
    Purification of ribonuclease T 1 by diethylaminoethylcellulose chromatography (1971), Biochem. J., 121, 591-596.
    View publication on PubMedView publication on EuropePMC
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA + H2O Aspergillus oryzae two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
diethylaminoethylcellulose chromatography Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation Taka-diastase, commercial product of Aspergillus oryzae Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA + H2O
-
 Aspergillus oryzae Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ?
RNA + H2O two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates Aspergillus oryzae ?
-
 ?