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Literature summary for 4.6.1.23 extracted from
- The action mode of the ribosome-inactivating protein alpha-sarcin (2000), J. Biomed. Sci., 7, 420-428.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of the genomic alpha-sarcin gene pGEM-T/FL-alpha plasmid, construction of the alpha-sarcin gene and its mutants by PCR, using genomic DNA as template | Aspergillus giganteus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C148S | point mutation constructed from cDNA clone | Aspergillus giganteus |
| C6S | point mutation constructed from cDNA clone | Aspergillus giganteus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| rRNA + H2O | Aspergillus giganteus | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus giganteus | - |
filamentous fungus, mold | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Aspergillus giganteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA + H2O | hydrolyzes naked RNA at purines in both single-and double-stranded regions | Aspergillus giganteus | ? | - |
? | |
| rRNA + H2O | - |
Aspergillus giganteus | ? | - |
? | |
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Release 2023.1 (January 2023)
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