Cloned (Comment) | Organism |
---|---|
cloned and expressed in Escherichia coli RB791 | Aspergillus giganteus |
Protein Variants | Comment | Organism |
---|---|---|
E96Q | mutagenesis variant protein | Aspergillus giganteus |
H137Q | mutagenesis variant protein | Aspergillus giganteus |
H50/137Q | mutagenesis variant protein | Aspergillus giganteus |
H50/137Q/E96Q | mutagenesis variant protein | Aspergillus giganteus |
H50Q | mutagenesis variant protein | Aspergillus giganteus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
ApA | pH 7.0, 37°C, mutant H50Q | Aspergillus giganteus | |
0.04 | - |
ApA | pH 7.0 or pH 5.0, 37°C | Aspergillus giganteus | |
0.045 | - |
ApA | pH 5.0, 37°C, mutant H50Q | Aspergillus giganteus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
rRNA + H2O | Aspergillus giganteus | - |
? | - |
? | |
rRNA + H2O | Aspergillus giganteus MDH 18894 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus giganteus | - |
MDH 18894 | - |
Aspergillus giganteus | - |
filamentous fungus, mold | - |
Aspergillus giganteus MDH 18894 | - |
MDH 18894 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Aspergillus giganteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ApA + H2O | - |
Aspergillus giganteus | 3'-AMP + adenosine | - |
? | |
ApA + H2O | - |
Aspergillus giganteus MDH 18894 | 3'-AMP + adenosine | - |
? | |
additional information | acts as a cyclizing ribonuclease | Aspergillus giganteus | ? | - |
? | |
additional information | acts as a cyclizing ribonuclease | Aspergillus giganteus MDH 18894 | ? | - |
? | |
rRNA + H2O | - |
Aspergillus giganteus | ? | - |
? | |
rRNA + H2O | - |
Aspergillus giganteus MDH 18894 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000014 | - |
ApA | pH 7.0, 37°C, mutant H50Q | Aspergillus giganteus | |
0.000016 | - |
ApA | pH 5.0, 37°C, mutant H50Q | Aspergillus giganteus | |
0.0001 | - |
ApA | pH 7.0, 37°C | Aspergillus giganteus | |
0.0002 | - |
ApA | pH 5.0, 37°C | Aspergillus giganteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus giganteus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8.5 | - |
Aspergillus giganteus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8.5 | - |
Aspergillus giganteus |