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Literature summary for 4.6.1.23 extracted from

  • Lacadena, J.; Mancheno, J.M.; Martinez-Ruiz, A.; Martinez del Pozo, A.; Gasset, M.; Onaderra, M.; Gavilanes, J.G.
    Substitution of histidine-137 by glutamine abolishes the catalytic activity of the ribosome-inactivating protein alpha-sarcin (1995), Biochem. J., 309, 581-586.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.23

Cloned(Commentary)

Cloned (Comment) Organism
cloned and expressed in Escherichia coli Aspergillus giganteus

Protein Variants

Protein Variants Comment Organism
E96A mutagenised using the T7-GEN TM in vitro mutagenesis kit, 80% less cytotoxity to HeLa cells Aspergillus giganteus
H137Q substitution abolishes the catalytic activity Aspergillus giganteus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus giganteus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Aspergillus giganteus
-
 ?
-
 ?
rRNA + H2O Aspergillus giganteus MDH 18894
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus giganteus
-
 MDH 18894
-
Aspergillus giganteus
-
 filamentous fungus, mold
-
Aspergillus giganteus MDH 18894
-
 MDH 18894
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aspergillus giganteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
28 S rRNA + H2O
-
 Aspergillus giganteus alpha-fragment from the 3' end of 28S rRNA
-
 ?
28 S rRNA + H2O
-
 Aspergillus giganteus MDH 18894 alpha-fragment from the 3' end of 28S rRNA
-
 ?
additional information alpha-sarcin site nucleotide sequence is identical in the large rRNAs of cytoplasmic ribosomes from a range of evolutionary diverse organisms, evolutionarily conserved in all eukaryotes and prokaryotes so far tested Aspergillus giganteus ?
-
 ?
additional information alpha-sarcin site nucleotide sequence is identical in the large rRNAs of cytoplasmic ribosomes from a range of evolutionary diverse organisms, evolutionarily conserved in all eukaryotes and prokaryotes so far tested Aspergillus giganteus MDH 18894 ?
-
 ?
rRNA + H2O
-
 Aspergillus giganteus ?
-
 ?
rRNA + H2O
-
 Aspergillus giganteus MDH 18894 ?
-
 ?