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Literature summary for 4.6.1.22 extracted from

  • Niranjanakumari, S.; Day-Storms, J.J.; Ahmed, M.; Hsieh, J.; Zahler, N.H.; Venters, R.A.; Fierke, C.A.
    Probing the architecture of the B. subtilis RNase P holoenzyme active site by cross-linking and affinity cleavage (2007), RNA, 13, 521-535.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.22

Cloned(Commentary)

Cloned (Comment) Organism
into the pET28b vector for expression in Escherichia coli BL21DE3pLysS cells Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
D15C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
D42C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
E40C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
H3C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
K64C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
N61C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
Q38C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
R45C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
R60C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
R62C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
R65C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
R68C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
R7C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis
V46C single-cysteine P protein mutant for cross-linking assay Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by CM-Sepharose ion-exchange chromatography and affinity chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information single-turnover kinetic assays are performed using 32P-labeled pre-tRNAAsp, containing a 14-nucleotide leader sequence, as a substrate Bacillus subtilis ?
-
 ?
pre-tRNAAsp + H2O
-
 Bacillus subtilis ?
-
 ?

Synonyms

Synonyms Comment Organism
Bacillus subtilis ribonuclease
-
 Bacillus subtilis
RNase P
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 single turnover assay Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.024
-
 pre-tRNAAsp R60C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.1
-
 pre-tRNAAsp R65C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.11
-
 pre-tRNAAsp R7C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.15
-
 pre-tRNAAsp D15C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.2
-
 pre-tRNAAsp N61C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.22
-
 pre-tRNAAsp R68C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.23
-
 pre-tRNAAsp K64C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.23
-
 pre-tRNAAsp wild-type, single turnover cleavage rate constant kobs Bacillus subtilis
0.24
-
 pre-tRNAAsp H3C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.26
-
 pre-tRNAAsp D42C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.26
-
 pre-tRNAAsp R62C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.27
-
 pre-tRNAAsp E40C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.27
-
 pre-tRNAAsp R45C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.27
-
 pre-tRNAAsp R45C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.27
-
 pre-tRNAAsp R60C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.27
-
 pre-tRNAAsp R7C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.28
-
 pre-tRNAAsp D15C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.28
-
 pre-tRNAAsp R65C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.28
-
 pre-tRNAAsp V46C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.29
-
 pre-tRNAAsp K64C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.3
-
 pre-tRNAAsp E40C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.3
-
 pre-tRNAAsp H3C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.3
-
 pre-tRNAAsp N61C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.31
-
 pre-tRNAAsp Q38C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.32
-
 pre-tRNAAsp V46C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.33
-
 pre-tRNAAsp Q38C mutant, single turnover cleavage rate constant kobs Bacillus subtilis
0.34
-
 pre-tRNAAsp R62C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.37
-
 pre-tRNAAsp D42C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue Bacillus subtilis
0.37
-
 pre-tRNAAsp R68C mutant, single turnover cleavage rate constant kobs Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 single turnover assay Bacillus subtilis