Cloned (Comment) | Organism |
---|---|
into the pET28b vector for expression in Escherichia coli BL21DE3pLysS cells | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D15C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
D42C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
E40C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
H3C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
K64C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
N61C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
Q38C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
R45C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
R60C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
R62C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
R65C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
R68C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
R7C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
V46C | single-cysteine P protein mutant for cross-linking assay | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by CM-Sepharose ion-exchange chromatography and affinity chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | single-turnover kinetic assays are performed using 32P-labeled pre-tRNAAsp, containing a 14-nucleotide leader sequence, as a substrate | Bacillus subtilis | ? | - |
? | |
pre-tRNAAsp + H2O | - |
Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bacillus subtilis ribonuclease | - |
Bacillus subtilis |
RNase P | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
single turnover assay | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
pre-tRNAAsp | R60C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.1 | - |
pre-tRNAAsp | R65C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.11 | - |
pre-tRNAAsp | R7C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.15 | - |
pre-tRNAAsp | D15C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.2 | - |
pre-tRNAAsp | N61C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.22 | - |
pre-tRNAAsp | R68C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.23 | - |
pre-tRNAAsp | K64C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.23 | - |
pre-tRNAAsp | wild-type, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.24 | - |
pre-tRNAAsp | H3C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.26 | - |
pre-tRNAAsp | D42C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.26 | - |
pre-tRNAAsp | R62C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.27 | - |
pre-tRNAAsp | E40C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.27 | - |
pre-tRNAAsp | R45C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.27 | - |
pre-tRNAAsp | R45C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.27 | - |
pre-tRNAAsp | R60C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.27 | - |
pre-tRNAAsp | R7C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.28 | - |
pre-tRNAAsp | D15C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.28 | - |
pre-tRNAAsp | R65C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.28 | - |
pre-tRNAAsp | V46C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.29 | - |
pre-tRNAAsp | K64C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.3 | - |
pre-tRNAAsp | E40C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.3 | - |
pre-tRNAAsp | H3C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.3 | - |
pre-tRNAAsp | N61C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.31 | - |
pre-tRNAAsp | Q38C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.32 | - |
pre-tRNAAsp | V46C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.33 | - |
pre-tRNAAsp | Q38C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis | |
0.34 | - |
pre-tRNAAsp | R62C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.37 | - |
pre-tRNAAsp | D42C mutant, single turnover cleavage rate constant kobs, protein contains an AOP modification at the cysteine residue | Bacillus subtilis | |
0.37 | - |
pre-tRNAAsp | R68C mutant, single turnover cleavage rate constant kobs | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
single turnover assay | Bacillus subtilis |