BRENDA - Enzyme Database show
show all sequences of 4.6.1.21

Peptides isolated from Enterobacter nuclease as potential polyamine binding sites

Frank, J.J.; Hawk, I.A.; Levy, C.C.; Biochim. Biophys. Acta 432, 369-380 (1976)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
putrescine
enhances activity against poly(C) and yeast RNA
Enterobacter sp.
spermidine
enhances activity against poly(C) and yeast RNA, reverses inhibition by poly(G) by removal of inhibitor from the enzymes surface
Enterobacter sp.
spermine
enhances activity against poly(C) and yeast RNA, reverses inhibition by poly(G) by removal of inhibitor from the enzymes surface
Enterobacter sp.
Application
Application
Commentary
Organism
analysis
-
Enterobacter sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
Poly(A)
-
Enterobacter sp.
Poly(G)
inhibition at a concentration of 0.25 nM is complete
Enterobacter sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
stimulating
Enterobacter sp.
Mg2+
stimulating
Enterobacter sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
RNA + H2O
Enterobacter sp.
preferential cleavage at CpA
nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates
-
Enterobacter sp.
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Enterobacter sp.
-
-
-
Purification (Commentary)
Commentary
Organism
-
Enterobacter sp.
Storage Stability
Storage Stability
Organism
-20°C
Enterobacter sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
RNA + H2O
preferential cleavage at CpA
134566
Enterobacter sp.
nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates
-
134566
Enterobacter sp.
?
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
putrescine
enhances activity against poly(C) and yeast RNA
Enterobacter sp.
spermidine
enhances activity against poly(C) and yeast RNA, reverses inhibition by poly(G) by removal of inhibitor from the enzymes surface
Enterobacter sp.
spermine
enhances activity against poly(C) and yeast RNA, reverses inhibition by poly(G) by removal of inhibitor from the enzymes surface
Enterobacter sp.
Application (protein specific)
Application
Commentary
Organism
analysis
-
Enterobacter sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Poly(A)
-
Enterobacter sp.
Poly(G)
inhibition at a concentration of 0.25 nM is complete
Enterobacter sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
stimulating
Enterobacter sp.
Mg2+
stimulating
Enterobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
RNA + H2O
Enterobacter sp.
preferential cleavage at CpA
nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates
-
Enterobacter sp.
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Enterobacter sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C
Enterobacter sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
RNA + H2O
preferential cleavage at CpA
134566
Enterobacter sp.
nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates
-
134566
Enterobacter sp.
?
Other publictions for EC 4.6.1.21
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
730982
Beck
CdiA from enterobacter cloacae ...
Enterobacter cloacae, Enterobacter cloacae ATCC 13047
Structure
22
707-718
2014
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1
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1
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2
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3
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2
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1
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1
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2
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2
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2
2
-
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-
134565
Karpetsky
Covalent linkage of poly(A) to ...
Enterobacter sp.
J. Biol. Chem.
255
2713-2721
1980
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-
-
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2
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3
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1
-
1
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2
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2
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3
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1
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2
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134566
Frank
Peptides isolated from Enterob ...
Enterobacter sp.
Biochim. Biophys. Acta
432
369-380
1976
3
1
-
-
-
-
2
-
-
2
-
1
-
1
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-
1
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1
1
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3
1
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2
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2
-
1
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-
1
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1
1
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-
134067
Krishna
On the localization of ribonuc ...
Enterobacter sp.
FEBS Lett.
29
105-108
1973
-
-
-
-
-
-
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1
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1
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1
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-
134567
Marotta
Preferred sites of digestion o ...
Enterobacter sp.
Biochemistry
12
2901-2904
1973
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1
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-
-
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1
-
1
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1
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1
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1
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1
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-
134568
Levy
Enterobacter ribonuclease. II. ...
Enterobacter sp.
Biochim. Biophys. Acta
246
476-486
1971
-
1
-
-
-
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-
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2
1
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2
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1
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2
1
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5
-
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1
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2
1
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1
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2
1
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5
-
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-
134569
Levy
Residue specificity of a ribon ...
Enterobacter sp.
J. Biol. Chem.
245
3257-3262
1970
-
1
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-
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2
-
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4
-
1
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1
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1
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2
1
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1
1
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1
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2
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4
-
1
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1
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2
1
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1
1
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