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Literature summary for 4.6.1.20 extracted from

  • Solivio, B.; Yu, N.; Addepalli, B.; Limbach, P.A.
    Improving RNA modification mapping sequence coverage by LC-MS through a nonspecific RNase U2-E49A mutant (2018), Anal. Chim. Acta, 1036, 73-79 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis improvement of RNA modification mapping sequence coverage by LC-MS. The non-specific ribonuclease activity RNase U2 E49A substitution mutant provides increased sequence coverage of substrate RNA during modification mapping. Data analysis software is modified to account for non-specific digestion. This combination allows efficient and accurate RNA modification mapping Ustilago sphaerogena

Protein Variants

Protein Variants Comment Organism
E49A mutant with non-specific ribonuclease activity and a tendency to undercut RNA Ustilago sphaerogena

Organism

Organism UniProt Comment Textmining
Ustilago sphaerogena P00654
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