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Literature summary for 4.6.1.20 extracted from
- Influence of key residues on the heterologous extracellular production of fungal ribonuclease U2 in the yeast Pichia pastoris (2009), Protein Expr. Purif., 65, 223-229.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris, different signal-peptide cleavage processing sites for different recombinant proteins | Ustilago sphaerogena |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C1S/C54S | lacking one disulfide bridge, 60% of wild-type activity | Ustilago sphaerogena |
| H101Q | devoid of detectable activity | Ustilago sphaerogena |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12590 | - |
mutant C1S/C54S, MALDI-TOF | Ustilago sphaerogena |
| 13030 | - |
wild type, MALDI-TOF | Ustilago sphaerogena |
| 13330 | - |
mutant H101Q, MALDI-TOF, posttranslational modification suggested | Ustilago sphaerogena |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ustilago sphaerogena | P00654 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ustilago sphaerogena |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Ustilago sphaerogena |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease U2 | - |
Ustilago sphaerogena |
| RNase U2 | - |
Ustilago sphaerogena |
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Release 2023.1 (January 2023)
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