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Literature summary for 4.6.1.20 extracted from

  • Martinez-Ruiz, A.; Garcia-Ortega, L.; Kao, R.; Onaderra, M.; Mancheno, J.M.; Davies, J.; Martinez del Pozo, A.; Gavilanes, J.G.
    Ribonuclease U2: cloning, production in Pichia pastoris and affinity chromatography purification of the active recombinant protein (2000), FEMS Microbiol. Lett., 189, 165-169.
    View publication on PubMed
Search for more literature for 4.6.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Pichia pastoris Ustilago sphaerogena

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 RNA 37°C, pH 4.5, recombinant enzyme Ustilago sphaerogena
0.23
-
 RNA 37°C, pH 4.5, fungal enzyme Ustilago sphaerogena

Organism

Organism UniProt Comment Textmining
Ustilago sphaerogena P00654 CBS 534.71
-
Ustilago sphaerogena CBS 534.71 P00654 CBS 534.71
-

Purification (Commentary)

Purification (Comment) Organism
purification of recombinant enzyme using several chromatographic steps, including affinity chromatography on 2´-5'-ADP-Sepharose Ustilago sphaerogena

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity increases 78fold after purification from crude extracts Ustilago sphaerogena

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA + H2O
-
 Ustilago sphaerogena 3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
 ?
RNA + H2O
-
 Ustilago sphaerogena CBS 534.71 3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates
-
 ?

Synonyms

Synonyms Comment Organism
RNase U2
-
 Ustilago sphaerogena