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Literature summary for 4.6.1.2 extracted from

  • Purohit, R.; Weichsel, A.; Montfort, W.R.
    Crystal structure of the alpha subunit PAS domain from soluble guanylyl cyclase (2013), Protein Sci., 22, 1439-1444.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
nitric oxide stimulates cyclase activity Manduca sexta

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Manduca sexta

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 1.4-1.6 M ammonium sulfate, 50 mM sodium cacodylate (pH 5.5-6.5) and 15 mM magnesium acetate tetrahydrate Manduca sexta

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Manduca sexta
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
-
 Manduca sexta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Manduca sexta
-
 3',5'-cyclic GMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Manduca sexta O77105
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Manduca sexta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
 Manduca sexta 3',5'-cyclic GMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
heterodimer
-
 Manduca sexta

Synonyms

Synonyms Comment Organism
sGC
-
 Manduca sexta
soluble guanylyl cyclase
-
 Manduca sexta

Cofactor

Cofactor Comment Organism Structure
heme
-
 Manduca sexta