Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | neither staurosporine nor Go6976 activate isoforms GC-A or GC-B | Homo sapiens | |
natriuretic peptide | - |
Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Go6976 | competitive inhibitor. Go6976 reduces GTP binding to the catalytic site of isoforms GC-A and GC-B. Inhibition of isoform GC-B is minimal in the absence of ATP and 1 mM ATP increases the inhibition 4fold | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Homo sapiens | 3',5'-cyclic-GMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GC-A | isoform | Homo sapiens |
GC-B | isoform | Homo sapiens |
guanylyl cyclase A | - |
Homo sapiens |
guanylyl cyclase B | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | ATP activates guanylyl cyclase isoforms GC-A and GC-B. 0.01 mM Go6976 increases the potency of ATP for isoform GC-B 4fold | Homo sapiens | |
heme | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
Go6976 | in the presence of 1 mM ATP, isozyme GC-A, in 25 mM HEPES (pH 7.4), 50 mM NaCl, 0.1% (w/v) bovine serum albumin, 0.5 mM isobutylmethyl xanthine, 1 mM EDTA, 0.5 mM microcystin and 5 mM MgCl2, at 37°C | Homo sapiens | |
0.0013 | - |
Go6976 | in the presence of 1 mM ATP, isozyme GC-B, in 25 mM HEPES (pH 7.4), 50 mM NaCl, 0.1% (w/v) bovine serum albumin, 0.5 mM isobutylmethyl xanthine, 1 mM EDTA, 0.5 mM microcystin and 5 mM MgCl2, at 37°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.002 | - |
in the presence of 1 mM ATP, isozyme GC-B, in 25 mM HEPES (pH 7.4), 50 mM NaCl, 0.1% (w/v) bovine serum albumin, 0.5 mM isobutylmethyl xanthine, 1 mM EDTA, 0.5 mM microcystin and 5 mM MgCl2, at 37°C | Homo sapiens | Go6976 |