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Literature summary for 4.6.1.2 extracted from

  • Ramanathan, S.; Mazzalupo, S.; Boitano, S.; Montfort, W.R.
    Thrombospondin-1 and angiotensin II inhibit soluble guanylyl cyclase through an increase in intracellular calcium concentration (2011), Biochemistry, 50, 7787-7799.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
BAY 41-2272 riociguat, allosteric stimulator Homo sapiens
nitric oxide
-
Homo sapiens
YC-1 allosteric stimulator Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ the enzyme is inhibited by high intracellular Ca2+ concentration Homo sapiens
additional information inhibition of soluble guanylyl cyclase requires binding to cell-surface receptor CD47. A thrombospondin-1 C-terminal fragment (E3CaG1) readily inhibits sGC in Jurkat T cells. This inhibition requires an increase in intracellular Ca+ concentration. Addition of angiotensin II also strongly inhibits soluble guanylyl cyclase activity by increasing intracellular Ca+ concentration Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.069
-
GTP in the absence of Ca2+ and in the presence of 0.05 mM nitric oxide, in 50 mM HEPES, pH 7.5, at 37°C Homo sapiens
0.234
-
GTP in the absence of Ca2+ and nitric oxide, in 50 mM HEPES, pH 7.5, at 37°C Homo sapiens
0.857
-
GTP in the presence of 2 mM Ca2+ and in the absence of nitric oxide, in 50 mM HEPES, pH 7.5, at 37°C Homo sapiens
0.887
-
GTP in the presence of 2 mM Ca2+ and 0.05 mM nitric oxide, in 50 mM HEPES, pH 7.5, at 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
JURKAT cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
Homo sapiens 3',5'-cyclic-GMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
sGC
-
Homo sapiens
soluble guanylate cyclase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme the enzyme possesses a heme-containing beta subunit Homo sapiens

General Information

General Information Comment Organism
physiological function soluble guanylate cyclase lowers intracellular Ca2+ concentration in response to nitric oxide, inducing vasodilationand is inhibited by high intracellular Ca2+ concentration, providing a fine balance between signals for vasodilation and vasoconstriction Homo sapiens