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Literature summary for 4.6.1.2 extracted from

  • Peshenko, I.V.; Olshevskaya, E.V.; Savchenko, A.B.; Karan, S.; Palczewski, K.; Baehr, W.; Dizhoor, A.M.
    Enzymatic properties and regulation of the native isozymes of retinal membrane guanylyl cyclase (RetGC) from mouse photoreceptors (2011), Biochemistry, 50, 5590-5600.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
guanylyl cyclase activating protein the native RetGC isozymes are accelerated 5-28fold at physiological concentrations of guanylyl cyclase activating proteins GCAP1 and GCAP2 Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) CodonPlus cells Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
 GTP isozyme RetGC2, stimulated by guanylyl cyclase activating protein 1,in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C Mus musculus
0.64
-
 GTP isozyme RetGC1, stimulated by guanylyl cyclase activating protein 1, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C Mus musculus
0.7
-
 GTP isozyme RetGC1, stimulated by guanylyl cyclase activating protein 2, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C Mus musculus
0.79
-
 GTP isozyme RetGC2, stimulated by guanylyl cyclase activating protein 2,in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C Mus musculus
1.55
-
 GTP nonstimulated isozyme RetGC1, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C Mus musculus
3.18
-
 GTP nonstimulated isozyme RetGC2, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mus musculus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
109000
-
 x * 109000, isozyme RetGC2, SDS-PAGE Mus musculus
112000
-
 x * 112000, isozyme RetGC1, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
photoreceptor
-
 Mus musculus
-
retina
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP isozyme RetGC1 contributes 23-28% to the maximal cGMP synthesis rate in mouse rod outer segments. The activity of isozyme RetGC2 is about 5times lower than isozyme RetGC1 Mus musculus 3',5'-cyclic-GMP + diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 109000, isozyme RetGC2, SDS-PAGE Mus musculus
? x * 112000, isozyme RetGC1, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
RetGC1 isozyme Mus musculus
RetGC2 isozyme Mus musculus
retinal membrane guanylyl cyclase
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
 GTP nonstimulated isozyme RetGC1, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C Mus musculus
20
-
 GTP nonstimulated isozyme RetGC2, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C Mus musculus
33
-
 GTP isozyme RetGC1, stimulated by guanylyl cyclase activating protein 2, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C Mus musculus
74
-
 GTP isozyme RetGC1, stimulated by guanylyl cyclase activating protein 1, in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C Mus musculus
94
-
 GTP isozyme RetGC2, stimulated by guanylyl cyclase activating protein 2,in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C Mus musculus
116
-
 GTP isozyme RetGC2, stimulated by guanylyl cyclase activating protein 1,in 30 mM MOPS-KOH (pH 7.2), 60 mM KCl, 4 mM NaCl, 1 mM dithiothreitol, 2 mM Ca2+/EGTA buffer, at 30°C Mus musculus

General Information

General Information Comment Organism
physiological function isozyme RetGC1 is absolutely required for cone function and survival Mus musculus