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Literature summary for 4.6.1.2 extracted from
- Crystal structure of the signaling helix coiled-coil domain of the beta1 subunit of the soluble guanylyl cyclase (2010), BMC Struct. Biol., 10, 2-2.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| nitric oxide | - |
Rattus norvegicus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the beta1 subunit of soluble guanylyl cyclase is expressed in Escherichia coli BL21 (DE3) pLysS cells | Rattus norvegicus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 0.1 M Bis-Tris pH 7.0, and 0.7 M ammonium sulfate | Rattus norvegicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19500 | - |
1 * 38500 + 1 * 19500, gel filtration, homodimers of sGC have also been observed but are not functionally active | Rattus norvegicus |
| 38500 | - |
1 * 38500 + 1 * 19500, gel filtration, homodimers of sGC have also been observed but are not functionally active | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | Rattus norvegicus | - |
3',5'-cyclic-GMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | P20595 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, Q-Sepharose column chromatography, and Superdex 75 gel filtration | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | - |
Rattus norvegicus | 3',5'-cyclic-GMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 38500 + 1 * 19500, gel filtration, homodimers of sGC have also been observed but are not functionally active | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sGC | - |
Rattus norvegicus |
| sGCbeta1 | beta1 subunit of soluble guanylyl cyclase | Rattus norvegicus |
| soluble guanylyl cyclase | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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