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Literature summary for 4.6.1.2 extracted from
- Purification and characterization of recombinant human soluble guanylate cyclase produced from baculovirus-infected insect cells (2009), Protein Expr. Purif., 65, 133-139.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-ethyl-3-(ethylaminoethyl)-1-hydroxy-2-oxo-1-triazene | i.e. NOC-12 | Homo sapiens |  |
| NO | 340fold activation | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged soluble guanylate cyclase in Spodoptera frugiperda SF9 cells using the baculovirus transfection method | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0498 | - |
GTP | pH 7.5, 37°C, NO-stimulated recombinant enzyme | Homo sapiens | |
| 0.0901 | - |
GTP | pH 7.5, 37°C, not stimulated recombinant enzyme | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
1 * 77000, alpha-subunit, + 1 * 70000, beta-subunit, SDS-PAGE | Homo sapiens |
| 77000 | - |
1 * 77000, alpha-subunit, + 1 * 70000, beta-subunit, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | Homo sapiens | - |
3',5'-cyclic GMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble guanylate cyclase from Spodoptera frugiperda SF9 cells by anion exchange chromatography, nickel affinity chromatography, and again anion exchange chromatography on a porus resin | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | - |
Homo sapiens | 3',5'-cyclic GMP + diphosphate | - |
? | |
| additional information | structure-function relationship, overview | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 77000, alpha-subunit, + 1 * 70000, beta-subunit, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| sGC | - |
Homo sapiens |
| soluble guanylate cyclase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
GTP | pH 7.5, 37°C, not stimulated recombinant enzyme | Homo sapiens | |
| 28.7 | - |
GTP | pH 7.5, 37°C, NO-stimulated recombinant enzyme | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | one heme per heterodimer | Homo sapiens | |
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