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Literature summary for 4.6.1.2 extracted from

  • Sun, L.; Wang, H.; Hu, J.; Han, J.; Matsunami, H.; Luo, M.
    Guanylyl cyclase-D in the olfactory CO2 neurons is activated by bicarbonate (2009), Proc. Natl. Acad. Sci. USA, 106, 2041-2046.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
bicarbonate activates cGMP-producing ability of guanylyl cyclase-D, a membrane guanylate cyclase exclusively expressed in the CO2-responsive olfactory sensory neurons, by directly acting on the intracellular cyclase domain of GC-D. The molecular mechanism for pH-independent GC-D activation is distinct from the release-from-repression model for other membrane guanylate cyclases, overview Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
functional transient expression of GC-D HEK-293T cell membranes, expression of His-tagged intracellular cyclase domain of GC-D consisting of GC-D residues 850-1110 in insect HiFive cells by using the baculovirus expression system Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mus musculus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP Mus musculus
-
 3',5'-cyclic GMP + diphosphate
-
 ?
additional information Mus musculus modeling of the intracellular signal transduction for CO2 detection by GC-D+ neurons and the molecular mechanism of bicarbonate sensing by GC-D, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GC-D partially from HEK-293T cell membranes, recombinant His-tagged intracellular cyclase domain of GC-D consisting of GC-D residues 850-1110 in insect HiFive cells by nickel affinity chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
olfactory sensory neuron the membrane guanylate cyclase is exclusively expressed in the CO2-responsive olfactory sensory neurons Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
 Mus musculus 3',5'-cyclic GMP + diphosphate
-
 ?
additional information modeling of the intracellular signal transduction for CO2 detection by GC-D+ neurons and the molecular mechanism of bicarbonate sensing by GC-D, overview Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
GC-D
-
 Mus musculus
guanylyl cyclase-D
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus