Literature summary for 4.6.1.2 extracted from

Maron, B.A.; Zhang, Y.Y.; Handy, D.E.; Beuve, A.; Tang, S.S.; Loscalzo, J.; Leopold, J.A.
Aldosterone increases oxidant stress to impair guanylyl cyclase activity by cysteinyl thiol oxidation in vascular smooth muscle cells (2009), J. Biol. Chem., 284, 7665-7672.

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Activating Compound

Activating Compound	Comment	Organism	Structure
NO	activates the soluble guanylyl cyclase	Bos taurus

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and C122A mutant enzymes in COS-7 cells	Bos taurus

Protein Variants

Protein Variants	Comment	Organism
C122A	site-directed mutagenesis of the aldosterone modification site, the mutant is insensitive to inhibition by aldosterone or H2O2	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
aldosterone	diminishes GC activity by activating NADPH oxidase in bovine aortic VSMC to increase ROS levels and induce oxidative posttranslational modification of Cys122, a beta1-subunit cysteinyl residue	Bos taurus
H2O2	diminishes GC activity by activating NADPH oxidase in bovine aortic VSMC to increase ROS levels and induce oxidative posttranslational modification of Cys122, a beta1-subunit cysteinyl residue	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Bos taurus	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GTP	Bos taurus	sGC produces cGMP, a second messenger required for normal vascular smooth muscle cell, VSMC, relaxation. Activating NADPH oxidase in bovine aortic VSMC increases ROS levels and induces oxidative posttranslational modification of Cys122, a beta1-subunit cysteinyl residue of the guanylate cyclase leading to its inhibition	3',5'-cyclic GMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
aorta	-	Bos taurus	-
blood vessel	-	Bos taurus	-
smooth muscle cell	vascular	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP	-	Bos taurus	3',5'-cyclic GMP + diphosphate	-	?
GTP	sGC produces cGMP, a second messenger required for normal vascular smooth muscle cell, VSMC, relaxation. Activating NADPH oxidase in bovine aortic VSMC increases ROS levels and induces oxidative posttranslational modification of Cys122, a beta1-subunit cysteinyl residue of the guanylate cyclase leading to its inhibition	Bos taurus	3',5'-cyclic GMP + diphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	the soluble guanylyl cyclase forms a heterodimer	Bos taurus

Synonyms

Synonyms	Comment	Organism
soluble guanylyl cyclase	-	Bos taurus

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Release 2023.1 (January 2023)