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Literature summary for 4.6.1.2 extracted from

  • Hodson, C.A.; Ambrogi, I.G.; Scott, R.O.; Mohler, P.J.; Milgram, S.L.
    Polarized apical sorting of guanylyl cyclase C is specified by a cytosolic signal (2006), Traffic, 7, 456-464.
    View publication on PubMed
Search for more literature for 4.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
atrial natriuretic peptide
-
 Rattus norvegicus
heat-stable enterotoxin
-
 Rattus norvegicus

Application

Application Comment Organism
additional information GCC is involved in cytosolic apical sorting signals in epithelial cells, apical sorting determinant for GCC is a region of 11 highly conserved amino acids (PTPPTVENQQR) in the COOH terminus, which are essential for the normal polarization of GCC in MDCK cells. This sequence is sufficient to selectively target an unpolarized reporter protein, interleukin-2 receptor alpha-chain, to the apical membrane Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
MDCK cell lines expressing HA-tagged GCC or HA-tagged GCC lacking the amino acids from the conserved 63 amino acid span Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information GCC lacking the amino acids from the conserved 63 amino acid span, localized in an unpolarized manner at both the apical and basolateral membranes, GCC protein lacking 32 or 52 amino acids from the COOH terminus, both mutations have no effect on the apical localization of GCC Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Rattus norvegicus 5829
-
membrane at the apical membrane GCA and GCC both present, at the basolateral membrane only GCA present, GCC is delivered directly to the apical membrane Rattus norvegicus 16020
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
 Rattus norvegicus 3',5'-cyclic GMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
GCA
-
 Rattus norvegicus
GCC
-
 Rattus norvegicus
guanylyl cyclase C
-
 Rattus norvegicus