Activating Compound | Comment | Organism | Structure |
---|---|---|---|
(Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate | sGC ferrous-nitrosyl complex adopts two 5-coordinate conformations, a lower activity closed form, which releases NO slowly, and a higher activity open form, which releases NO rapidly | Rattus norvegicus | |
3-(5'-hydroxymethyl-3'-furyl)-1-benzylindazole | - |
Rattus norvegicus |
Application | Comment | Organism |
---|---|---|
additional information | sGC has two NO-regulated activity states | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
sGC beta1 cDNA inserted between the NotI and XbaI sites of the plasmid pFastBac1, H6sGC alpha1 gene inserted between the NotI and XbaI sites of pFastBac1, expression in Sf9 cells | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on Ni2+ column and anion-exchange column | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanosine-5'-[alpha,beta-methylene]triphosphate | - |
Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sGC | - |
Rattus norvegicus |
soluble guanylate cyclase | - |
Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 37 | inclusion of 3-(5'-hydroxymethyl-3'-furyl)-1-benzylindazole moderately accelerates NO dissociation from sGC and beta2(1-217) at 37°C and dramatically accelerates NO dissociation from sGC at 10°C. Presence of guanosine-5'-[alpha,beta-methylene]triphosphate also dramatically accelerates NO dissociation from sGC at 10 °C | Rattus norvegicus |