Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.2 extracted from

  • Martin, E.; Berka, V.; Bogatenkova, E.; Murad, F.; Tsai, A.L.
    Ligand selectivity of soluble guanylyl cyclase: effect of the hydrogen-bonding tyrosine in the distal heme pocket on binding of oxygen, nitric oxide, and carbon monoxide (2006), J. Biol. Chem., 281, 27836-27845.
    View publication on PubMed
Search for more literature for 4.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
5-cyclopropyl-2-[1-(2-fluorobenzyl)-1H-pyrazolo[3,4-b]pyridin-3-yl]-pyrimidin-4-ylamine
-
 Caldanaerobacter subterraneus subsp. tengcongensis
CO limited affinity Caldanaerobacter subterraneus subsp. tengcongensis
additional information no affinity for O2, the mechanism of oxygen exclusion by sGC not only involves the lack of hydrogen bonding in the distal heme pocket, but also depends on structural elements from other domains of sGC Caldanaerobacter subterraneus subsp. tengcongensis
NO
-
 Caldanaerobacter subterraneus subsp. tengcongensis
protoporphyrin IX
-
 Caldanaerobacter subterraneus subsp. tengcongensis

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant in Sf9 cells Caldanaerobacter subterraneus subsp. tengcongensis

Protein Variants

Protein Variants Comment Organism
I145Y substitution in the full-length beta-subunit of the sGC heterodimer, does not produce an oxygen-binding enzyme, but impedes the association of NO and destabilizes the NO-heme complex. The tyrosine in the distal heme pocket impedes both the binding and dissociation of the CO ligand. Mutation does not affect stimulatory effect of manganese ion on the activity of the enzyme, thus function of the catalytic domain is not directly affected Caldanaerobacter subterraneus subsp. tengcongensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Caldanaerobacter subterraneus subsp. tengcongensis

Organism

Organism UniProt Comment Textmining
Caldanaerobacter subterraneus subsp. tengcongensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration and on nickel-agarose column, at least 95% pure Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
 Caldanaerobacter subterraneus subsp. tengcongensis 3',5'-cyclic GMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
sGC
-
 Caldanaerobacter subterraneus subsp. tengcongensis
soluble guanylyl cyclase
-
 Caldanaerobacter subterraneus subsp. tengcongensis