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Literature summary for 4.6.1.2 extracted from

  • Karmakar, S.; Ukil, A.; Mukherjee, S.; Das, P.K.
    Regulation of guanylyl cyclase by intracellular Ca2+ in relation to the infectivity of the protozoan parasite, Leishmania donovani (2006), Int. J. Biochem. Cell Biol., 38, 1277-1289.
    View publication on PubMed
Search for more literature for 4.6.1.2

Activating Compound

Activating Compound Comment Organism Structure
additional information insensitive to NO stimulation, bovine calmodulin fails to stimulate the enzyme Leishmania donovani

Application

Application Comment Organism
medicine guanylyl cyclase activity is related to intracellular Ca2+ mobilization and this Ca2+-cGMP cross-talk may in turn be associated with parasite infectivity Leishmania donovani

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) as a fusion protein with an N-terminal polyhistidine tag after subcloning into bacterial expression vector pET16b Leishmania donovani

Inhibitors

Inhibitors Comment Organism Structure
1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin 1-one decreases infectivity of promastigotes Leishmania donovani
6-anilino-quinoline-5-8-quinone decreases infectivity of promastigotes Leishmania donovani
additional information native and recombinant enzyme unaffected by chlorpromazine and trifluoprazine Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.386
-
 GTP purified enzyme Leishmania donovani
0.455
-
 GTP recombinant enzyme Leishmania donovani

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Leishmania donovani 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dose-dependent stimulation Leishmania donovani
Mg2+
-
 Leishmania donovani
Mn2+ the recombinant enzyme shows substantially low level of activity when Mg2+ is replaced with Mn2+ Leishmania donovani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106000
-
 sequence analysis Leishmania donovani

Organism

Organism UniProt Comment Textmining
Leishmania donovani Q8I7Z5 MHOM/IN/1983/AG83
-
Leishmania donovani MHOM/IN/1983/AG83 Q8I7Z5 MHOM/IN/1983/AG83
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography Leishmania donovani

Source Tissue

Source Tissue Comment Organism Textmining
amastigote
-
 Leishmania donovani
-
promastigote
-
 Leishmania donovani
-

Storage Stability

Storage Stability Organism
-70°C, 50 mM HEPES buffer, pH 7.6, 1 mM DTT, 100 mM NaCl, 5 mM EDTA, 50% glycerol Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP
-
 Leishmania donovani 3',5'-cyclic GMP + diphosphate
-
 ?
GTP
-
 Leishmania donovani MHOM/IN/1983/AG83 3',5'-cyclic GMP + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
soluble guanylyl cyclase
-
 Leishmania donovani