Literature summary for 4.6.1.2 extracted from

Huang, S.H.; Rio, D.C.; Marletta, M.A.
Ligand binding and inhibition of an oxygen-sensitive soluble guanylate cyclase, Gyc-88E, from Drosophila (2007), Biochemistry, 46, 15115-15122.

Search for more literature for 4.6.1.2

Application

Application	Comment	Organism
additional information	biochemical properties of Gyc-88E are unique for guanylate cyclases, the enzyme possibly functions as an oxygen sensor	Drosophila melanogaster

Cloned(Commentary)

Cloned (Comment)	Organism
Gyc-88E(1-597) expressed in S2 cells as a polyoma-tagged construct under the control of the metallothionein promoter	Drosophila melanogaster

Inhibitors

Inhibitors	Comment	Organism	Structure
CO	forms stable complexes with Gyc-88E(1-597), inhibits 2.9fold	Drosophila melanogaster
NO	forms stable complexes with Gyc-88E(1-597), inhibits 2fold	Drosophila melanogaster
O2	forms stable complexes with Gyc-88E(1-597), inhibits 3.2fold	Drosophila melanogaster

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.66	-	GTP	with 0.273 mM oxygen	Drosophila melanogaster
0.82	-	GTP	under anaerobic conditions	Drosophila melanogaster

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	Gyc-88E(1-597) is a Fe(II)-O2 complex	Drosophila melanogaster

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
143000	-	gel filtration	Drosophila melanogaster

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Gyc-88E(1-597) purified to more than 95% purity, by gel filtration and on anion-exchange column	Drosophila melanogaster

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.6	-	Fe(II)-unligated Gyc-88E(1-597)	Drosophila melanogaster

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP	-	Drosophila melanogaster	3',5'-cyclic GMP + diphosphate	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 x 68000, SDS-PAGE	Drosophila melanogaster

Synonyms

Synonyms	Comment	Organism
Gyc-88E	-	Drosophila melanogaster
sGC	-	Drosophila melanogaster
soluble guanylate cyclase	-	Drosophila melanogaster

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.051	-	O2	-	Drosophila melanogaster

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Release 2023.1 (January 2023)