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Literature summary for 4.6.1.2 extracted from
- Characterization of two unusual guanylyl cyclases from Dictyostelium (2002), J. Biol. Chem., 277, 9167-9174.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GTPgammaS | activates by reducing the Km for GTP | Dictyostelium discoideum |  |
| GTPgammaS | activates by reducing the Km for GTP, half-maximal activation at 0.008 mM | Dictyostelium sp. | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 0.00005 mM, 50% inhibition | Dictyostelium discoideum | |
| Ca2+ | 0.0002 mM, 50% inhibition | Dictyostelium sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
GTP | 22°C, pH 8.0 | Dictyostelium discoideum | |
| 0.41 | - |
GTP | 22°C, pH 8.0 | Dictyostelium sp. | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Dictyostelium discoideum | 16020 | - |
| soluble | - |
Dictyostelium sp. | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal activity at 1-2 mM, 5fold lower activity than with Mn2+ | Dictyostelium sp. | |
| Mg2+ | predominantly active with Mg2+/GTP | Dictyostelium discoideum | |
| Mn2+ | maximal activity at 1-2 mM | Dictyostelium sp. | |
| Mn2+ | supports only 30% of Mg2+-dependent activity | Dictyostelium discoideum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | Dictyostelium discoideum | - |
3',5'-cyclic GMP + diphosphate | - |
? | |
| GTP | Dictyostelium sp. | - |
3',5'-cyclic GMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium discoideum | - |
during growth, 20-40% of GC activity is attributed to GCA | - |
| Dictyostelium sp. | - |
during growth, 60-80% of GC activity is attributed to sGC | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | - |
Dictyostelium discoideum | 3',5'-cyclic GMP + diphosphate | - |
? | |
| GTP | - |
Dictyostelium sp. | 3',5'-cyclic GMP + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GCA | - |
Dictyostelium discoideum |
| guanylyl cyclase A | - |
Dictyostelium discoideum |
| sGC | - |
Dictyostelium sp. |
| soluble guanylyl cyclase | - |
Dictyostelium sp. |
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