Literature summary for 4.6.1.19 extracted from

Kriz, M.; Snasel, J.; Kopecky, V.; Pav, O.; Rosenberg, I.; Stepanek, J.
Structural changes of human RNase L upon homodimerization investigated by Raman spectroscopy (2012), Biochim. Biophys. Acta, 1824, 1039-1044.

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Activating Compound

Activating Compound	Comment	Organism	Structure
2'-5'-linked oligoadenylates	natural 2'-5'-linked tetraadenylates and three tetramers with 3'-end AMP units replaced with ribo-, arabino- and xylo-configured phosphonate analogues of AMP are enzyme activators with equal potency. The enzyme is activated by the binding of 2'-5'-linked oligoadenylates to the N-terminal ankyrin repeat domain, which causes the inactive monomer to form a catalytically active homodimer	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography and dialysis	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
leukemia cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	assay substrate is 32P-labeled synthetic substrate r(C11U2C7)	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	active enzyme form	Homo sapiens
More	the estimated secondary structural composition of inactive monomeric RNase L is 44% alpha-helix, 28% beta-sheet, 17% beta-turns and 11% of unordered structures, whereas dimerization causes a slight decrease in alpha-helix and increase in beta-sheet (ca. 2%) content. Enzyme dimerization affects at least three Tyr, five Phe and two Trp residues. The alpha-beta structural switch may fix domain positions in the hinge region (residues ca. 336-363) during homodimer formation, Raman spectroscopy, overview	Homo sapiens

Synonyms

Synonyms	Comment	Organism
RNase L	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	key enzyme in the defense system	Homo sapiens

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Release 2023.1 (January 2023)