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Literature summary for 4.6.1.19 extracted from
- A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding (2005), FEBS J., 272, 363-374.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D209N | inactivates the enzyme without affecting RNA binding | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for catalysis | Escherichia coli |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mutant D209N and wild-type | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly-8 adenylic acid | pH 8, 1 mM MgCl2, incubation at 30°C for 5 min | Escherichia coli | AMP + ? | - |
? | |
| poly-8 adenylic acid | pH 8, 1 mM MgCl2, incubation at 30°C for 5 min | Escherichia coli JM109 | AMP + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease II | - |
Escherichia coli |
| RNase II | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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