Literature summary for 4.6.1.19 extracted from

Ohgi, K.; Takeuchi, M.; Iwama, M.; Irie, M.
Enzymatic properties of mutant enzymes at Trp49 and Tyr57 of RNase Rh from Rhizopus niveus (1996), J. Biochem., 119, 9-15.

Search for more literature for 4.6.1.19

Protein Variants

Protein Variants	Comment	Organism
W49A	greatly increased activity	Rhizopus niveus
W49F	16% activity of native enzyme	Rhizopus niveus
W49F	higher preference toward pyrimidine bases	Rhizopus niveus
W49I	greatly increased activity	Rhizopus niveus
W49Y	greatly increased activity	Rhizopus niveus
Y57A	more active toward RNA and less active toward XpGs	Rhizopus niveus
Y57F	greatly increased activity	Rhizopus niveus
Y57G	more active toward RNA and less active toward XpGs	Rhizopus niveus
Y57K	more active toward RNA and less active toward XpGs	Rhizopus niveus
Y57M	more active toward RNA and less active toward XpGs	Rhizopus niveus
Y57V	more active toward RNA and less active toward XpGs	Rhizopus niveus
Y57W	greatly increased activity	Rhizopus niveus
Y57W	higher preference toward purine bases	Rhizopus niveus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	for different mutants	Rhizopus niveus

Organism

Organism	UniProt	Comment	Textmining
Rhizopus niveus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
mutant proteins	Rhizopus niveus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
RNA + H2O	-	Rhizopus niveus	3'-phosphomononucleotides	-	?

Synonyms

Synonyms	Comment	Organism
RNase Rh	-	Rhizopus niveus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)