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Literature summary for 4.6.1.19 extracted from
- Preparation and properties of RNase T2 immobilized on concanavalin A-sepharose (1989), Appl. Biochem. Biotechnol., 22, 237-246.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | EDTA activates soluble enzyme, no effect on immobilized | Aspergillus oryzae |
General Stability
| General Stability | Organism |
|---|---|
| stability of immobilized enzyme | Aspergillus oryzae |
| urea, 8 M, soluble and immobilized enzyme, stable | Aspergillus oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Aspergillus oryzae |  |
| Zn2+ | - |
Aspergillus oryzae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | RNA: 555 mg/ml soluble enzyme, 266 mg/ml immobilized enzyme | Aspergillus oryzae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | activates immobilized enzyme, inhibits soluble | Aspergillus oryzae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
immmobilized enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Aspergillus oryzae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | Takadiastase powder | Aspergillus oryzae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA + H2O | - |
Aspergillus oryzae | 3'-phosphomononucleotides | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 62 | - |
soluble and immobilized enzyme | Aspergillus oryzae |
| 65 | - |
- |
Aspergillus oryzae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | 40°C: about 60% of activity maximum, soluble and immobilized enzyme, 70°C: about 70% immobilized enzyme, 40% soluble enzyme, of activity maximum | Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 4.5, 1 h, soluble enzyme: about 29% loss of activity, immobilized enzyme: about 10% loss of activity | Aspergillus oryzae |
| 70 | - |
pH 4.5, 1 h, soluble form: complete loss of activity, immobilized form: about 40% loss of activity | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
soluble and immobilized enzyme | Aspergillus oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 5.5 | pH 3.5, 5.5: about 70% of activity maximum, soluble and immobilized enzyme | Aspergillus oryzae |
| 3.5 | 6 | pH 3.5: 80% of activity maximum, soluble and immobilized form, pH 6: soluble form 100%, immobilized form 90% of maximum acitivity | Aspergillus oryzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
1 h, about 20% loss of activity, soluble and immobilized form | Aspergillus oryzae |
| 4.5 | 5.5 | 1 h | Aspergillus oryzae |
| 6 | - |
1 h, about 10% loss of activity | Aspergillus oryzae |
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