Crystallization (Comment) | Organism |
---|---|
enzyme as dimer and tetramer after three-dimensional domain swapping, hanging-drop vapour-diffusion method, mixing of 1.6 mg/ml protein in 0.1 M sodium citrate buffer, pH 6.5, and 0.3 M NaCl, with reservoir solution containing 22% w/v PEG 8000, 0.1 M ammonium sulfate, X-ray diffraction structure determination and analysis at 2.70 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of five residues in the loop connecting the N-terminal helix to the core of monomeric human pancreatic ribonuclease leads to the formation of an enzymatically active domain-swapped dimer. Three-dimensional domain swapping can be a mechanism for the formation of elaborate large assemblies in which the protein, apart from the swapping, retains its original fold | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
sulfate | 13 sulfate anions are identified in the electrondensity map of the two dimers in the asymmetric unit of the crystal, confirming that this ion plays a fundamental role in the crystallization process. Four sulfate ions are positioned at the active sites, as typically observed in several other members of the pancreatic-like superfamily, the remaining anions are located on positive patches of the rod surface | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07998 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Homo sapiens | - |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Homo sapiens |
More | deletion of five residues in the loop connecting the N-terminal helix to the core of monomeric human pancreatic ribonuclease leads to the formation of an enzymatically active domain-swapped dimer. Domain-swapped dimer fibrils can form in solution. Two dimers in the asymmetric unit of the crystal: twofold symmetry of the dimers with a very strong inter-dimer association, a composite active site is generated by residues belonging to subunits A and B of one of the two dimers in the asymmetric unit | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
pancreatic RNase | - |
Homo sapiens |