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Literature summary for 4.6.1.18 extracted from
- Functional role of glutamine 28 and arginine 39 in double stranded RNA cleavage by human pancreatic ribonuclease (2011), PLoS ONE, 6, e17159.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G38D | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
| additional information | all RNase A oligomers obtained either by cross-linking reactions or by one of the other ways by which the enzyme protein can aggregate, acquire the ability to degrade double-stranded polyribonucleotides | Homo sapiens |
| Q28A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
| Q28A/G38D | site-directed mutagenesis, the mutant shows reduced activity and thermal stability compared to the wild-type enzyme | Homo sapiens |
| Q28A/G38D/R39A | site-directed mutagenesis, the mutant shows highly reduced activity and thermal stability compared to the wild-type enzyme | Homo sapiens |
| Q28A/R39A | site-directed mutagenesis, the mutant shows reduced activity and thermal stability compared to the wild-type enzyme | Homo sapiens |
| Q28L | site-directed mutagenesis, the mutant shows enhanced activity compared to the wild-type enzyme | Homo sapiens |
| Q28L/R39A | site-directed mutagenesis, the mutant shows reduced activity and thermal stability compared to the wild-type enzyme | Homo sapiens |
| R39A | site-directed mutagenesis, the mutant shows reduced activity and thermal stability compared to the wild-type enzyme | Homo sapiens |
| R39A/G38D | site-directed mutagenesis, the mutant shows highly reduced activity and thermal stability compared to the wild-type enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michelis-Menten curves and catalytic efficiencies of HPR and its mutant variants on different substrates | Homo sapiens | |
| 0.0115 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28L | Homo sapiens | |
| 0.0135 | - |
poly(A)-poly(U) | pH 7.5, 37°C, wild-type enzyme | Homo sapiens | |
| 0.0338 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28A/G38D | Homo sapiens | |
| 0.034 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant G38D | Homo sapiens | |
| 0.0409 | - |
poly(C) | pH 7.5, 37°C, mutant Q28A/G38D/R39A | Homo sapiens |  |
| 0.0435 | - |
poly(C) | pH 7.5, 37°C, mutant Q28L/R39A | Homo sapiens | |
| 0.048 | - |
poly(C) | pH 7.5, 37°C, mutant G38D | Homo sapiens | |
| 0.0485 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant R39A | Homo sapiens | |
| 0.052 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28A | Homo sapiens | |
| 0.0566 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28L/R39A | Homo sapiens | |
| 0.0567 | - |
poly(C) | pH 7.5, 37°C, mutant R39A | Homo sapiens | |
| 0.0572 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28A/R39A | Homo sapiens | |
| 0.0609 | - |
poly(C) | pH 7.5, 37°C, mutant R39A/G38D | Homo sapiens | |
| 0.0739 | - |
poly(C) | pH 7.5, 37°C, mutant Q28A | Homo sapiens | |
| 0.0804 | - |
poly(C) | pH 7.5, 37°C, mutant Q28L | Homo sapiens | |
| 0.0823 | - |
poly(C) | pH 7.5, 37°C, wild-type enzyme | Homo sapiens | |
| 0.0853 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28A/G38D/R39A | Homo sapiens | |
| 0.091 | - |
poly(C) | pH 7.5, 37°C, mutant Q28A/G38D | Homo sapiens | |
| 0.0993 | - |
poly(C) | pH 7.5, 37°C, mutant Q28A/R39A | Homo sapiens | |
| 0.1059 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant R39A/G38D | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme shows high activity on double stranded RNA | Homo sapiens | ? | - |
? | |
| poly(A)-poly(U) + H2O | - |
Homo sapiens | ? | - |
? | |
| poly(C) + H2O | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme is a member of RNase A superfamily | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
thermal denaturation profiles of the wild-type enzyme and its mutant variants, overview | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.57 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant R39A/G38D | Homo sapiens | |
| 2.23 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28A/G38D/R39A | Homo sapiens | |
| 4.85 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28A/G38D | Homo sapiens | |
| 5.35 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant R39A | Homo sapiens | |
| 5.525 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28A/R39A | Homo sapiens | |
| 7.001 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28A | Homo sapiens | |
| 8.315 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant G38D | Homo sapiens | |
| 8.89 | - |
poly(A)-poly(U) | pH 7.5, 37°C, wild-type enzyme | Homo sapiens | |
| 12.27 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28L | Homo sapiens | |
| 14.71 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28L/R39A | Homo sapiens | |
| 1033 | - |
poly(C) | pH 7.5, 37°C, mutant Q28A/G38D | Homo sapiens | |
| 1100 | - |
poly(C) | pH 7.5, 37°C, mutant Q28A/G38D/R39A | Homo sapiens | |
| 1233 | - |
poly(C) | pH 7.5, 37°C, mutant Q28L/R39A | Homo sapiens | |
| 1500 | - |
poly(C) | pH 7.5, 37°C, mutant Q28A/R39A | Homo sapiens | |
| 1650 | - |
poly(C) | pH 7.5, 37°C, mutant R39A/G38D | Homo sapiens | |
| 1667 | - |
poly(C) | pH 7.5, 37°C, mutant G38D | Homo sapiens | |
| 1833 | - |
poly(C) | pH 7.5, 37°C, mutant Q28L | Homo sapiens | |
| 2167 | - |
poly(C) | pH 7.5, 37°C, mutant Q28A | Homo sapiens | |
| 2317 | - |
poly(C) | pH 7.5, 37°C, wild-type enzyme | Homo sapiens | |
| 2833 | - |
poly(C) | pH 7.5, 37°C, mutant R39A | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | arginine 39 is crucial for the dsRNA melting activity, and Gly38 is required, both these residues are not directly involved in the RNA cleavage activity | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 21.7 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28A/G38D/R39A | Homo sapiens | |
| 28.3 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant R39A/G38D | Homo sapiens | |
| 96.7 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28A/R39A | Homo sapiens | |
| 110 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant R39A | Homo sapiens | |
| 135 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28A | Homo sapiens | |
| 143.3 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28A/G38D | Homo sapiens | |
| 245 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant G38D | Homo sapiens | |
| 260 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28L/R39A | Homo sapiens | |
| 658.3 | - |
poly(A)-poly(U) | pH 7.5, 37°C, wild-type enzyme | Homo sapiens | |
| 1067 | - |
poly(A)-poly(U) | pH 7.5, 37°C, mutant Q28L | Homo sapiens | |
| 15170 | - |
poly(C) | pH 7.5, 37°C, mutant Q28A/R39A | Homo sapiens | |
| 18170 | - |
poly(C) | pH 7.5, 37°C, mutant R39A | Homo sapiens | |
| 20170 | - |
poly(C) | pH 7.5, 37°C, mutant Q28A/G38D | Homo sapiens | |
| 20670 | - |
poly(C) | pH 7.5, 37°C, mutant Q28L | Homo sapiens | |
| 24830 | - |
poly(C) | pH 7.5, 37°C, mutant Q28A | Homo sapiens | |
| 26830 | - |
poly(C) | pH 7.5, 37°C, mutant Q28A/G38D/R39A | Homo sapiens | |
| 27170 | - |
poly(C) | pH 7.5, 37°C, mutant R39A/G38D | Homo sapiens | |
| 28170 | - |
poly(C) | pH 7.5, 37°C, wild-type enzyme | Homo sapiens | |
| 28330 | - |
poly(C) | pH 7.5, 37°C, mutant Q28L/R39A | Homo sapiens | |
| 40670 | - |
poly(C) | pH 7.5, 37°C, mutant G38D | Homo sapiens | |
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