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Literature summary for 4.6.1.18 extracted from
- Zero-length dimers of ribonuclease A: further characterization and no evidence of cytotoxicity (2010), Bioconjug. Chem., 21, 635-645.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E9A | site-directed mutagenesis | Bos taurus |
| K66A | site-directed mutagenesis, no intramolecular bonds form in the K66A variant | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of the covalent RNase A oligomers by two-step cation exchange chromatography and two gel filtration steps | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Bos taurus | - |
| pancreas | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzymatic activities of the various RNase A monomeric and zero-length or domain-swapped oligomeric species on single-stranded yeast or double-stranded poly(A)-poly(U) RNA, overview. The dimers show higher activity compared to the monomners | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(A)-poly(U) + H2O | the enzyme dimers degrade poly(A)-poly(U) dsRNA with an activity that increases with the increase of the oligomer's basicity | Bos taurus | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | besides dimers, also trimers and higher oligomers can be identified among the products of the covalently linking reaction, and the zero-length dimers appear not to be a unique species, but heterogeneous, overview. Quantification of the zero-length oligomers | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease A | - |
Bos taurus |
| RNase A | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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