Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21 | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
D53A | slight stabilization, increases the helix propensity of alpha-helix 3 | Bos taurus |
F120A | destabilization | Bos taurus |
F46A | equivalent destabilization for F46A and F46L variants | Bos taurus |
F46L | equivalent destabilization for F46A and F46L variants | Bos taurus |
F8A | substitution of Phe8 results in a recombinant variant significantly destabilized | Bos taurus |
F8L | substitution of Phe8 results in a recombinant variant significantly destabilized | Bos taurus |
K7A | slight stabilization, increases the helix propensity of alpha-helix 1 | Bos taurus |
L35A | less destabilizing than M30A substitution | Bos taurus |
L51A | shortening the side chain of the hydrophobic solvent-exposed residue Leu51 to Ala has almost no effect in the stability of ribonuclease A | Bos taurus |
M13A | critical position for the ribonuclease A stability | Bos taurus |
M30A | critical position for the ribonuclease A stability | Bos taurus |
M79A | a minor role in the stabilization of the protein | Bos taurus |
Q28A | promotes an increase in the helix propensity, from 0.99 in the wild-type to 1.5 | Bos taurus |
T87A | no effect in the protein stability | Bos taurus |
V43A | substitution of the hydrophobic residue valin 43 by alanin results in an increase in the global stability of the ribonuclease A structure of 4.02 kJ/mol in free energy | Bos taurus |
Y25A | critical position for the ribonuclease A stability | Bos taurus |
Y25F | decreases the stability of the enzyme by 7.41 kJ/mol | Bos taurus |
Y73A | a minor role in the stabilization of the protein | Bos taurus |
Y97A | substitution of Tyr97 results in a variant significantly destabilized | Bos taurus |
Y97L | substitution of Tyr97 results in a variant significantly destabilized | Bos taurus |
General Stability | Organism |
---|---|
calculation of thermodynamic parameters from thermally induced unfolding curves | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13590 | - |
mutant enzyme Y73A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13590 | - |
mutant enzyme Y97A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13600 | - |
mutant enzyme Y25A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13610 | - |
mutant enzyme F120A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13610 | - |
mutant enzyme F46A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13610 | - |
mutant enzyme F8A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13620 | - |
mutant enzyme K7A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13620 | - |
mutant enzyme M13A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13620 | - |
mutant enzyme M30A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13620 | - |
mutant enzyme Q28A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13620 | - |
mutant enzyme T87A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13630 | - |
mutant enzyme Y97L, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13640 | - |
mutant enzyme D53A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13640 | - |
mutant enzyme L35A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13640 | - |
mutant enzyme L51A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13650 | - |
mutant enzyme F46L, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13650 | - |
mutant enzyme F8L, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13650 | - |
mutant enzyme M79A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13660 | - |
mutant enzyme V43A, MALDI-TOF/TOF mass spectrometry | Bos taurus |
13670 | - |
mutant enzyme Y25F, MALDI-TOF/TOF mass spectrometry | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P61823 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|
Synonyms | Comment | Organism |
---|---|---|
ribonuclease A | - |
Bos taurus |
RNase A | - |
Bos taurus |