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Literature summary for 4.6.1.18 extracted from
- The effect of additional disulfide bonds on the stability and folding of ribonuclease A (2009), Biophys. Chem., 141, 21-28.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A4C/V118C | site-directed mutagenesis | Bos taurus |
| H105C/V124C | site-directed mutagenesis | Bos taurus |
| I107C/A122C | site-directed mutagenesis | Bos taurus |
| M30C/N44C | site-directed mutagenesis | Bos taurus |
| R10C/R33C | site-directed mutagenesis | Bos taurus |
| V43C/R85C | site-directed mutagenesis | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | P61823 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-carboxyfluorescein-dArUdAdA-6-carboxytetramethylrhodamine + H2O | - |
Bos taurus | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribonuclease A | - |
Bos taurus |
| RNase A | - |
Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
The thermal stability of ribonuclease A and its variants is investigated by circular dichroism spectroscopy measurements. Thermal unfolding proves to be reversible and follows a two-state transition model as judged from the fit of the data. While H105C/V124C-ribonuclease A, the variant with the disulfide bond homolog to onconase is as stable as ribonuclease A and A4C/V118C- and V43C/R85C-ribonuclease A are more stable than ribonuclease A, R10C/R33C-, I107C/A122C-, and particularly M30C/N44C-ribonuclease A are destabilized in comparison to ribonuclease A | Bos taurus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
6-carboxyfluorescein-dArUdAdA-6-carboxytetramethylrhodamine | mutant enzyme M30C/N44C | Bos taurus | |
| 1044 | - |
6-carboxyfluorescein-dArUdAdA-6-carboxytetramethylrhodamine | mutant enzyme I107C/A122C | Bos taurus | |
| 1566 | - |
6-carboxyfluorescein-dArUdAdA-6-carboxytetramethylrhodamine | mutant enzyme H105C/V124C | Bos taurus | |
| 3132 | - |
6-carboxyfluorescein-dArUdAdA-6-carboxytetramethylrhodamine | mutant enzyme A4C/V118C | Bos taurus | |
| 3480 | - |
6-carboxyfluorescein-dArUdAdA-6-carboxytetramethylrhodamine | mutant enzyme V43C/R85C | Bos taurus | |
| 4002 | - |
6-carboxyfluorescein-dArUdAdA-6-carboxytetramethylrhodamine | mutant enzyme R10C/R33C | Bos taurus | |
| 17400 | - |
6-carboxyfluorescein-dArUdAdA-6-carboxytetramethylrhodamine | wild-type enzyme | Bos taurus | |
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Release 2023.1 (January 2023)
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