Protein Variants | Comment | Organism |
---|---|---|
C87A/C104A | 66% of wild-type activity, decrease in melting temperature | Lithobates pipiens |
F28A | 61% of wild-type activity, decrease in melting temperature | Lithobates pipiens |
F28T | 60% of wild-type activity, decrease in melting temperature | Lithobates pipiens |
F36Y | 80% of wild-type activity, decrease in melting temperature | Lithobates pipiens |
Glp1E | 41% of wild-type activity, decrease in melting temperature | Lithobates pipiens |
glp1P | 44% of wild-type activity, decrease in melting temperature | Lithobates pipiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lithobates pipiens | P22069 | - |
- |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the extremely high thermodynamic stability of enzyme is due to a dramatic deceleration of the unfolding reaction | Lithobates pipiens |
69.4 | - |
mutant C87A/C104A, melting temperature | Lithobates pipiens |
77.4 | - |
mutant F28T, melting temperature | Lithobates pipiens |
78.1 | - |
mutant F28A, melting temperature | Lithobates pipiens |
79.2 | - |
mutant F36Y, melting temperature | Lithobates pipiens |
87 | - |
mutant Glp1E, melting temperature | Lithobates pipiens |
87.7 | - |
mutant Glp1P, melting temperature | Lithobates pipiens |
88.5 | - |
wild-type, melting temperature | Lithobates pipiens |