Literature summary for 4.6.1.18 extracted from

Gaur, D.; Batra, J.K.
Role of aspartic acid 121 in human pancreatic ribonuclease catalysis (2005), Mol. Cell. Biochem., 275, 95-101.

Search for more literature for 4.6.1.18

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant enzymes in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D121A	Km-value for poly(C) is 2.4fold higher than wild-type value, Km-value for yeast tRNA is 1.25fold higher than wild-type value, turnover-number for poly(C) is 16.5fold lower than wild-type value, turnover-number for yeast tRNA is 1.3fold lower than wild-type value. ASp121 is crucial for the catalytic activity and may be involved in the depolymerization activity of the enzyme	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.7	-	poly(C)	pH 7.5, 37°C, wild-type enzyme	Homo sapiens
4	-	tRNA	pH 7.5, 37°C, wild-type enzyme	Homo sapiens
4	-	poly(C)	pH 7.5, 37°C, mutant enzyme D121A	Homo sapiens
5	-	tRNA	pH 7.5, 37°C, mutant enzyme D121A	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
poly (C) + H2O	-	Homo sapiens	3'-CMP + 3'-phosphooligonucleotides	-	?
tRNA + H2O	tRNA from yeast	Homo sapiens	3'-phosphomononucleotides + 3'-phosphooligonucleotides	-	?

Synonyms

Synonyms	Comment	Organism
HPR	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Homo sapiens

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Release 2023.1 (January 2023)