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Literature summary for 4.6.1.18 extracted from

  • Tanimizu, N.; Ueno, H.; Hayashi, R.
    Replacement of His12 or His119 of bovine pancreatic ribonuclease A with acidic amino acid residues for the modification of activity and stability (2002), J. Biosci. Bioeng., 94, 39-44.
    View publication on PubMed
Search for more literature for 4.6.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bos taurus

Protein Variants

Protein Variants Comment Organism
H119D with little effect on thermal stability Bos taurus
H12D with lower thermal stability than the wild type enzyme Bos taurus
H12E with lower thermal stability than the wild type enzyme Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 cytidinyl-3',5'-adenosine H119D mutant, pH 5.5, 25°C Bos taurus
0.55
-
 cytidine-2',3'-cyclic monophosphate H12E mutant, pH 5.5, 25°C Bos taurus
0.62
-
 cytidine-2',3'-cyclic monophosphate wild type enzyme, pH 5.5, 25°C Bos taurus
0.67
-
 cytidinyl-3',5'-adenosine wild type enzyme, pH 5.5, 25°C Bos taurus
0.71
-
 cytidinyl-3',5'-adenosine H12D mutant, pH 5.5, 25°C Bos taurus
0.74
-
 cytidinyl-3',5'-adenosine H12E mutant, pH 5.5, 25°C Bos taurus
3.9
-
 cytidine-2',3'-cyclic monophosphate H12D mutant, pH 5.5, 25°C Bos taurus
4
-
 cytidine-2',3'-cyclic monophosphate H119D mutant, pH 5.5, 25°C Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 bovine
-

Purification (Commentary)

Purification (Comment) Organism
chromatography on Mono S column Bos taurus

Reaction

Reaction Comment Organism Reaction ID
an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] pyrimidine-specific endoribonuclease which cleaves 3',5'-phosphodiester bonds of single strand RNA via transphosphorylation and subsequent hydrolysis reactions Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytidine-2',3'-cyclic monophosphate + H2O hydrolysis reaction Bos taurus 3'-CMP
-
 ?
cytidinyl-3',5'-adenosine + H2O CpA, transphosphorylation reaction Bos taurus adenosine + 3'-CMP
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 His12 mutants enzymes show low thermal stability, with a Tm of 45ºC. Wild type enzyme and His119 mutant show a Tm of 61ºC Bos taurus