Literature summary for 4.6.1.18 extracted from

Chatani, E.; Hayashi, R.
Functional and structural roles of constituent amino acid residues of bovine pancreatic ribonuclease A (2001), J. Biosci. Bioeng., 92, 98-107.

Search for more literature for 4.6.1.18

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
13680	-	amino acid sequence	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P61823	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]	the enzyme is composed of 124 amino acid residues. The amino acid residues involved in catalysis are His12, His119 and Lys41. The enzyme has six substrate binding subsites. One of them, B1, binds only to pyrimidine nucleotides and prefers to bind cytidine rather than uridine. Thr45 of the B1 subsite contributes to the enzyme substrate specificity and plays an important role in catalysis. Phe120 in the B1 subsite enhances substrate binding with the aid of its pi electron and hydrophobicity. Asn71 in the B2 subsite is responsible for productive interactions leading to efficient activity, and Glu111 in the same subsite contributes to substrate binding only in the binding of guanosine. Lys7, Arg10 and Gln11 in the P2 subsite also play an important role in catalysis. Some amino acid residues are located in the vicinity of the catalytic residues and contribute to the catalytic reaction by interacting with catalytic residues. Lys7, Arg10 and Lys66 maintain the optimum pKa of His12 and His119. Asp121 positions the proper tautomer of His119. Phe120 is responsible for the strict positioning of His119, and contributes to conformational stability. Tyr97 is involved in maintaining the correct position of Lys41. Cys65-Cys72 are located close to the catalytic and substrate binding residues and contribute to the proper spatial alignment of these residues	Bos taurus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
pancreas	-	Bos taurus	-

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Release 2023.1 (January 2023)