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Literature summary for 4.6.1.18 extracted from
- Conformational stability is a determinant of ribonuclease A cytotoxicity (2000), J. Biol. Chem., 275, 17463-17467.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bos taurus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G88R | mutant with similar thermal stability to wild type enzyme | Bos taurus |
| additional information | A4C/G88R/V118C mutant, with a new disulfide bond that links the N and C termini, is more stable to thermal denaturation than wild type and G88R enzymes. The conformational stability of the C40A/G88R/C95A and C65A/C72A/G88R mutants is less than that of G88R. A4C/C65A/C72A/G88R/V118C mutant, with a new disulfide bond is more stable than C65A/C72A/G88R mutant | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ribonuclease inhibitor | - |
Bos taurus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | P61823 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Bos taurus | - |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00024 | - |
ribonuclease inhibitor | G88R mutant, pH 6.0, 25ºC | Bos taurus | |
| 0.00035 | - |
ribonuclease inhibitor | C40A/G88R/C95A mutant, pH 6.0, 25ºC | Bos taurus | |
| 0.00065 | - |
ribonuclease inhibitor | A4C/G88R/V118C mutant, pH 6.0, 25ºC | Bos taurus | |
| 0.00078 | - |
ribonuclease inhibitor | C65A/C72A/G88R mutant, pH 6.0, 25ºC | Bos taurus | |
| 0.0039 | - |
ribonuclease inhibitor | A4C/C65A/C72A/G88R/V118C mutant, pH 6.0, 25ºC | Bos taurus | |
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Release 2023.1 (January 2023)
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