Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G89R/S90R | PE5 variant | Homo sapiens |
Q37R/R39D/K66R/N67G/G68D/G89R/S90G/R91D | PI5 variant | Homo sapiens |
R31E/K66R/N67G/G68D/R91A | PE3I2 variant | Homo sapiens |
R31E/Q37R/R39D/R91A | PE3I1 variant | Homo sapiens |
R31E/R91A | PE3 variant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ribonuclease inhibitor | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration on a Mono S HR 5/5 FPLC column | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytidine-2',3'-cyclic monophosphate + H2O | hydrolysis reaction | Homo sapiens | 3'-CMP | - |
? | |
poly(C) + H2O | - |
Homo sapiens | 3'-CMP + 3'-phosphooligonucleotides | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
all substitutions produce a decrease in the thermal stability of the variants | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00042 | - |
ribonuclease inhibitor | PE3 variant, pH 5.5, 25ºC | Homo sapiens | |
0.00077 | - |
ribonuclease inhibitor | PE3I2 variant, pH 5.5, 25ºC | Homo sapiens | |
0.0008 | - |
ribonuclease inhibitor | PE3I1 variant, pH 5.5, 25ºC | Homo sapiens | |
0.00134 | - |
ribonuclease inhibitor | PI5 variant, pH 5.5, 25ºC | Homo sapiens |