Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.6.1.18 extracted from

  • Bosch, M.; Benito, A.; Ribo, M.; Puig, T.; Beaumelle, B.; Vilanova, M.
    A nuclear localization sequence endows human pancreatic ribonuclease with cytotoxic activity (2004), Biochemistry, 43, 2167-2177.
    View publication on PubMed
Search for more literature for 4.6.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
G89R/S90R PE5 variant Homo sapiens
Q37R/R39D/K66R/N67G/G68D/G89R/S90G/R91D PI5 variant Homo sapiens
R31E/K66R/N67G/G68D/R91A PE3I2 variant Homo sapiens
R31E/Q37R/R39D/R91A PE3I1 variant Homo sapiens
R31E/R91A PE3 variant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ribonuclease inhibitor
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration on a Mono S HR 5/5 FPLC column Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytidine-2',3'-cyclic monophosphate + H2O hydrolysis reaction Homo sapiens 3'-CMP
-
 ?
poly(C) + H2O
-
 Homo sapiens 3'-CMP + 3'-phosphooligonucleotides
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 all substitutions produce a decrease in the thermal stability of the variants Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00042
-
 ribonuclease inhibitor PE3 variant, pH 5.5, 25ºC Homo sapiens
0.00077
-
 ribonuclease inhibitor PE3I2 variant, pH 5.5, 25ºC Homo sapiens
0.0008
-
 ribonuclease inhibitor PE3I1 variant, pH 5.5, 25ºC Homo sapiens
0.00134
-
 ribonuclease inhibitor PI5 variant, pH 5.5, 25ºC Homo sapiens