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Literature summary for 4.6.1.18 extracted from

  • Fujii, T.; Doi, Y.; Ueno, H.; Hayashi, R.
    Effect of mutagenic replacement of the carboxyl terminal amino acid, val124, on the properties and regeneration of bovine pancreatic ribonuclease A (2000), J. Biochem., 127, 877-881.
    View publication on PubMed
Search for more literature for 4.6.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutants in Escherichia coli Bos taurus

Protein Variants

Protein Variants Comment Organism
V124A C-terminus involved in the formation of disulfide bonds during refolding process Bos taurus
V124E C-terminus involved in the formation of disulfide bonds during refolding process Bos taurus
V124G C-terminus involved in the formation of disulfide bonds during refolding process Bos taurus
V124K C-terminus involved in the formation of disulfide bonds during refolding process Bos taurus
V124L C-terminus involved in the formation of disulfide bonds during refolding process Bos taurus
V124W C-terminus involved in the formation of disulfide bonds during refolding process Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.46 0.71 poly(C) wild type and mutant enzyme Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RNA + H2O Bos taurus endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the mutant proteins Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclic 2',3'-nucleoside monophosphate + H2O second step of hydrolysis is irreversible Bos taurus 3'-phosphomononucleotides
-
 ir
poly (C) + H2O
-
 Bos taurus 3'-CMP + 3'-phosphooligonucleotides
-
 ?
RNA + H2O endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates, e.g. tRNA, 18S and 28S rRNA, yeast RNA,4.5S RNA Bos taurus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?
RNA + H2O first step of hydrolysis is reversible Bos taurus cyclic 2',3'-nucleoside monophosphate
-
 r