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Literature summary for 4.6.1.17 extracted from

  • Hover, B.M.; Tonthat, N.K.; Schumacher, M.A.; Yokoyama, K.
    Mechanism of pyranopterin ring formation in molybdenum cofactor biosynthesis (2015), Proc. Natl. Acad. Sci. USA, 112, 6347-6352.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.17

Protein Variants

Protein Variants Comment Organism
D128A less than 1% of wild-type activity Escherichia coli
D17A about 30% of wild-type activity Escherichia coli
E112A less than 1% of wild-type activity Escherichia coli
E114A less than 1% of wild-type activity Escherichia coli
H77A less than 1% of wild-type activity Escherichia coli
K131A less than 1% of wild-type activity Escherichia coli
K21A about 18% of wild-type activity Escherichia coli
K51A less than 1% of wild-type activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00021
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate His-tagged wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli
0.00025
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli
0.001
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant K51A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
0.0016
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant H77A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
0.0024
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant E112A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
0.0043
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant E114A, pH not specified in the publication, temperature not specified in the publication Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A738
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate
-
 Escherichia coli cyclic pyranopterin phosphate + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
cyclic pyranopterin monophosphate synthase accessory protein
-
 Escherichia coli
MoaC
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00002
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant K51A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
0.00004
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant E114A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
0.00004
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant H77A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
0.00005
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate mutant E112A, pH not specified in the publication, temperature not specified in the publication Escherichia coli
0.0088
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli
0.0093
-
 (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate His-tagged wild-type, pH not specified in the publication, temperature not specified in the publication Escherichia coli

General Information

General Information Comment Organism
physiological function the physiological function of MoaA is the conversion of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate (GTP 3?,8-cyclase), and that of MoaC is to catalyze the rearrangement of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate into cyclic pyranopterin Escherichia coli