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Literature summary for 4.6.1.17 extracted from
- Overexpression, purification, crystallization and preliminary X-ray analysis of putative molybdenum cofactor biosynthesis protein C (MoaC2) from Mycobacterium tuberculosis H37Rv (2012), Acta Crystallogr. Sect. F, 68, 687-691.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Rv0864, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.003 ml of 10.5 mg/ml protein solution with 0.0015 ml reservoir solution containing 0.025 M potassium sodium tartrate tetrahydrate, pH 8.0, and equilibration against 1 ml reservoir solution, 15 days, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement calculations | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17500 | - |
x * 17500, MoaC2 | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | Mycobacterium tuberculosis | - |
cyclic pyranopterin phosphate + diphosphate | - |
? |  |
| GTP | Mycobacterium tuberculosis H37Rv | - |
cyclic pyranopterin phosphate + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WJR7 | gene Rv0864 | - |
| Mycobacterium tuberculosis H37Rv | P9WJR7 | gene Rv0864 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography and gel filtration | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | - |
Mycobacterium tuberculosis | cyclic pyranopterin phosphate + diphosphate | - |
? | |
| GTP | - |
Mycobacterium tuberculosis H37Rv | cyclic pyranopterin phosphate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 17500, MoaC2 | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MoaC2 | - |
Mycobacterium tuberculosis |
| molybdenum cofactor biosynthesis protein C | - |
Mycobacterium tuberculosis |
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