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Literature summary for 4.6.1.16 extracted from

  • Calvin, K.; Xue, S.; Ellis, C.; Mitchell, M.H.; Li, H.
    Probing the catalytic triad of an archaeal RNA splicing endonuclease (2008), Biochemistry, 47, 13659-13665.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.6.1.16

Cloned(Commentary)

Cloned (Comment) Organism
-
 Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
H257A residue of the catalytic triad, 28-fold reduction in activity Archaeoglobus fulgidus
H257C residue of the catalytic triad Archaeoglobus fulgidus
H257D residue of the catalytic triad Archaeoglobus fulgidus
H257N residue of the catalytic triad Archaeoglobus fulgidus
K287E residue of the catalytic triad, no activity in absence of divalent metal ions, activity observed in the presence of specific divalent metal ions Archaeoglobus fulgidus
K287Q residue of the catalytic triad Archaeoglobus fulgidus
K287R residue of the catalytic triad Archaeoglobus fulgidus
Y246F residue of the catalytic triad, 7-fold reduction in activity Archaeoglobus fulgidus
Y246F/H257A residues of the catalytic triad, no activity Archaeoglobus fulgidus
Y246H residue of the catalytic triad Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
 pretRNA wild-type protein, substrate: 5'-AAGCGACCGACC*AUA*GCUGCA-3' or 5'-AAUGCAGCGGUC*AAA*GGUCGC-3' Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-AAGCGACCGACC*AUA*GCUGCA-3' + H2O
-
 Archaeoglobus fulgidus ?
-
 ?
5'-AAUGCAGCGGUC*AAA*GGUCGC-3' + H2O
-
 Archaeoglobus fulgidus ?
-
 ?
pretRNA
-
 Archaeoglobus fulgidus ?
-
 ?

Synonyms

Synonyms Comment Organism
RNA splicing endonuclease
-
 Archaeoglobus fulgidus