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Literature summary for 4.6.1.16 extracted from

  • Xue, S.; Calvin, K.; Li, H.
    RNA recognition and cleavage by a splicing endonuclease (2006), Science, 312, 906-910.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
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Reaction

Reaction Comment Organism Reaction ID
pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus substrate recognition and binding structure and mechanism, catalytic mechanism, the catalytic triad is formed by His257, Lys287, and Tyr246, overview Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme cleaves two phosphodiester bonds within folded precursor RNAs during intron removal producing functional RNAs required for protein synthesis, the enzyme binds to bulge-helix-bulge motifs of RNA containing a noncleaved and a cleaved splice site, the enzyme the dimer recognizes cooperatively a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line cleavage reaction Archaeoglobus fulgidus ?
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Subunits

Subunits Comment Organism
dimer structure of the enzyme-BHB RNA complex, PDB code 2A9L, the dimer recognizes cooperatively a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line cleavage reaction Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
RNA splicing endonuclease
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Archaeoglobus fulgidus
splicing endonuclease
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Archaeoglobus fulgidus